In this study, 11 plant extracts from Bangladesh were used to evaluate the total phenolic and flavonoid content, in vitro antioxidant activities using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azinobis-(3-ethylbenzthiazolin-6-sulfonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) assay. Also, the inhibitory effect of nitric oxide (NO) production in RAW 264.7 macrophage cell line and the neuroprotective effect on H2O2-induced PC12 cells were tested. Our results revealed that Piper betle L. showed the highest total phenolic content (162.2 mg GAE/g extract) among the 11 plants from Bangladesh. Most plants showed strong radical scavenging effects and ferric reducing antioxidant power. Besides, Piper betle L. protected PC12 neuronal cells against H2O2 related oxidative stress in LPS-induced PC12 cells. Regarding the antiinflammatory effect, Piper betle L. significantly inhibited NO accumulation in LPS-induced RAW 264.7 cells. Our results provide evidence that Piper betle L. could be useful for the development of functional health foods.

Abstract
I. 서 론
II. 연구내용 및 방법
    1. 식물 추출물
    2. 시약 및 재료
    3. 총 페놀 함량 측정
    4. 총 플라보노이드 함량 측정
    5. DPPH 라디칼 소거능 측정
    6. ABTS 라디칼 소거능 측정
    7. 환원력 측정
    8. 세포 배양
    9. 세포독성 측정
    10. NO 생성 저해 능력 측정
    11. 산화스트레스에 대한 신경세포 보호효과
    12. 통계처리
III. 결과 및 고찰
    1. 총 페놀 및 플라보노이드 함량
    2. DPPH 라디칼 소거능
    3. ABTS 라디칼 소거능
    4. Ferric reducing antioxidant power (FRAP)
    5. 항산화 활성을 통한 신경세포 보호효과
    6. NO 생성 억제효과
IV. 요약 및 결론
감사의 글
Conflict of Interest
References

• 유소현(덕성여자대학교 바이오헬스융합학과) | SoHyeon You (Bio-Health Convergence Major, Duksung Women’s University)
• 김건희(덕성여자대학교 바이오헬스융합학과, 식품영양학과) | Gun-Hee Kim (Bio-Health Convergence Major, Department of Food and Nutrition, Duksung Women’s University) Corresponding author