논문 상세보기

생약복합물 용매추출물과 조다당획분의 생리활성 KCI 등재

The Physiological Activity of Crude Polysaccharide Solvent Extracted from Herbal Medicine Mixture

  • 언어KOR
  • URLhttps://db.koreascholar.com/Article/Detail/405478
구독 기관 인증 시 무료 이용이 가능합니다. 4,200원
한국식품영양학회지 (The Korean Journal of Food And Nutrition)
한국식품영양학회 (The Korean Society of Food and Nutrition)
초록

After ethanol (BM-E and RW-E) and hot-water (BM-HW and RW-HW) extracts were fractionated from two herbal mixtures (BM and RW), their physiological activities were investigated. All extracts consisted of more than 50% of neutral sugar, with their total polyphenol levels higher than flavonoid levels. Radical scavenging activities of EtOH extracts remained significantly higher compared to that of hot-water extracts, and in particular, RW-E showed consistently higher antioxidant activity than BM-E. When anti-inflammatory activities of the extracts were evaluated by LPS-stimulated RAW 264.7 cells at 10~500 μg/mL non-cytotoxicity doses, BM-E showed significantly higher levels of TNF-α, IL-1β, IL-6, and nitric oxide inhibitory activity than those of hot-water extracts and RW-E. Murine peritoneal macrophage cells were shown to be enhanced in crude polysaccharides (BM-CP and RW-CP fractionated from BM-HW and RW-HW) compared to hot-water extracts and polysaccharide K (PSK, positive control). Especially, RW-CP exhibited higher activity than BM-CP, and component sugar analysis showed that BM-CP mainly contained galacturonic acid, glucose, arabinose, galactose, and xylose (34.5%, 33.9%, 16.1%, 7.1%, and 6.3%, respectively), whereas RW-CP showed different measurements (29.5%, 59.2%, 5.0%, 4.5%, and 0.2%). In conclusion, two herbal mixtures could contain varying sets of physiological activities dependent on different extraction and fractionation methods.

목차
Abstract
서 론
재료 및 방법
    1. 재료 및 방법
    2. 생약복합물 용매추출물 및 조다당획분 분획
    3. 생약복합물 용매추출물의 구성분 및 조다당획분의 구성당 분석
    4. 생약복합물 용매추출물의 항산화
    5. 생약복합물 용매추출물의 항염증
    6. 생약복합물 용매추출물의 대식세포 자극활성
    7. 통계처리
결과 및 고찰
    1. 생약복합물의 용매추출물
    2. 생약복합물 용매추출물의 항산화 활성
    3. 생약복합물의 항염증 활성
    4. 생약복합물의 면역증진 활성
    5. 면역활성 조다당획분의 구성당 분석
요약 및 결론
References
저자
  • 신현영(고려대학교 의생명융합과학과) | Hyun-Young Shin (Dept. of Integrated Biomedical and Life Science, Korea University)
  • 김훈(경희대학교 피부생명공학센터) | Hoon Kim (Skin Biotechnology Center, Kyung Hee University)
  • 신지영((주)코시스바이오) | Ji-Young Shin (Cosis-Bio Co. Ltd.)
  • 이수정(경기대학교 식품생물공학전공) | Sue Jung Lee (Major in Food Science and Biotechnology, Kyonggi University)
  • 유광원(한국교통대학교 식품영양학전공) | Kwang-Won Yu (Major in Food and Nutrition, Korea National University of Transportation) Corresponding author