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pp.36-46
After ethanol (BM-E and RW-E) and hot-water (BM-HW and RW-HW) extracts were fractionated from two herbal mixtures (BM and RW), their physiological activities were investigated. All extracts consisted of more than 50% of neutral sugar, with their total polyphenol levels higher than flavonoid levels. Radical scavenging activities of EtOH extracts remained significantly higher compared to that of hot-water extracts, and in particular, RW-E showed consistently higher antioxidant activity than BM-E. When anti-inflammatory activities of the extracts were evaluated by LPS-stimulated RAW 264.7 cells at 10~500 μg/mL non-cytotoxicity doses, BM-E showed significantly higher levels of TNF-α, IL-1β, IL-6, and nitric oxide inhibitory activity than those of hot-water extracts and RW-E. Murine peritoneal macrophage cells were shown to be enhanced in crude polysaccharides (BM-CP and RW-CP fractionated from BM-HW and RW-HW) compared to hot-water extracts and polysaccharide K (PSK, positive control). Especially, RW-CP exhibited higher activity than BM-CP, and component sugar analysis showed that BM-CP mainly contained galacturonic acid, glucose, arabinose, galactose, and xylose (34.5%, 33.9%, 16.1%, 7.1%, and 6.3%, respectively), whereas RW-CP showed different measurements (29.5%, 59.2%, 5.0%, 4.5%, and 0.2%). In conclusion, two herbal mixtures could contain varying sets of physiological activities dependent on different extraction and fractionation methods.
서 론
재료 및 방법
1. 재료 및 방법
2. 생약복합물 용매추출물 및 조다당획분 분획
3. 생약복합물 용매추출물의 구성분 및 조다당획분의 구성당 분석
4. 생약복합물 용매추출물의 항산화
5. 생약복합물 용매추출물의 항염증
6. 생약복합물 용매추출물의 대식세포 자극활성
7. 통계처리
결과 및 고찰
1. 생약복합물의 용매추출물
2. 생약복합물 용매추출물의 항산화 활성
3. 생약복합물의 항염증 활성
4. 생약복합물의 면역증진 활성
5. 면역활성 조다당획분의 구성당 분석
요약 및 결론
References
