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Pectolinarin 고함유 곤드레 미세분말의 항산화 및 항비만 활성 KCI 등재

Antioxidant and Anti-Obesity Properties of Pectolinarin-rich Cirsium setidens Nakai Fine Powder

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한국식품영양학회지 (The Korean Journal of Food And Nutrition)
한국식품영양학회 (The Korean Society of Food and Nutrition)
초록

The aim of this study was to evaluate the basic data of Cirsium setidens Nakai fine powder (FPC), which will then be used in the development of functional fooditems. We measures and evaluated the level of pectolinarin content, phenol content, flavonoids content, antioxidants and anti-obesity properties of FPC. Our results from the study showed that the pectolinarin, phenol, and flavonoids contents of FPC measured at 10.95±0.15 mg/g, 12.92±0.18 mg gallic acid equivalent (GAE)/g and 26.47±0.77 mg rutin equivalent (RE)/g, respectively. The exhibited antioxidant activity of FPC increased significantly depending on the dosage, and additionally. FPC did not show any cytotoxicity up to the dosage level of 500 μg/mL. During adipocyte differentiation, FPC significantly inhibited ROS production and lipid accumulation, compared with the control substance. These results suggest that FPC could be considered a promising resource of natural antioxidants and could serve a variety of health-improving roles in the production of functional food ingredients.

목차
Abstract
서 론
재료 및 방법
    1. 실험재료 및 시약
    2. Pectolinarin 분석
    3. 총 페놀 및 총 플라보노이드 함량 측정
    4. DPPH radical 소거활성 평가
    5. ABTS radical 소거활성 평가
    6. FRAP assay
    7. Reducing power
    8. 세포독성평가(XTT assay)
    9. Oil Red O staining을 통한 지방축적량
    10. NBT assay를 이용한 ROS 함량 측정
    11. Western blot 분석
    12. 통계처리
결과 및 고찰
    1. 곤드레 미세분말의 pectolinarin 함량, 총 페놀 및 총플라보노이드 함량
    2. 곤드레 미세분말의 항산화능 측정
    3. 항산화 성분과 항산화 활성의 상관관계 분석
    4. 곤드레 미세분말의 세포독성
    5. 3T3-L1 세포내 지방 축적 및 ROS 생성에 대한 곤드레미세분말의 효과
    6. 3T3-L1 세포내 주요 바이오마커 변화에 미치는 곤드레 미세분말의 효과
요약 및 결론
References
저자
  • 박성진(한림성심대학교 관광외식조리과) | Sung-Jin Park (Dept. of Tourism Food Service Cuisine, Hallym Polytechnic University) Corresponding author
  • 김현덕((주)찬누리라이프) | Hyun-Duk Kim (Channuri Life Co. Ltd.)
  • 이윤환((주)찬누리라이프) | Yoon-Hwan Lee (Channuri Life Co. Ltd.)