Fenbendazole (FBZ) is one of the safest anthelmintic drugs. FBZ has been found to have anti-cancer effects by destabilizing microtubules. In this study, a synergistic effect of paclitaxel (PA), a microtubule-stabilizing anti-cancer agent, and FBZ was investigated on HL-60 cells, a human leukemia cell line. The metabolic activity of cells significantly decreased and the nucleus morphology upon the treatment of FBZ and PA based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assay and Hoechst 33342 staining, respectively. To investigate the involvement of reactive oxygen species (ROS), the metabolic activity of the cells after treatment of N-acetyl-L-cysteine (NAC) was measured. Indeed, NAC significantly increased the metabolic activity of the cells treated with FBZ and PA, suggesting that both drugs affect at least in part via ROS. Furthermore, FBZ and PA increased cell death in an annexin V- fluorescein isothiocyanate/propidium iodide staining assay. Taken together, FBZ and PA have a synergistic anti-cancer activity on HL-60 cells at a certain concentration. These results may provide researchers and clinicians in cancer-related fields with some valuable information to broaden the use of FBZ.

서 론
재료 및 방법
    세포와 물질 처리
    세포의 대사 활성도 측정
    유세포 분석
    Hoechst 33342를 이용한 세포의 핵 염색
    통계분석
결 과
    FBZ과 PA의 농도별 처리에 따른 대사 활성도의 변화
    FBZ와 PA를 처리한 HL-60세포에서 ROS의 효과 분석
    FBZ과 PA 처리에 따른 미토콘드리아막전위와 세포사 관찰
    Hoechst 33342 염색을 통한 세포핵 분석
고 찰
REFERENCES

• Ji Yun Sung(Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University)
• Hong-Gu Joo(Laboratory of Veterinary Pharmacology, College of Veterinary Medicine Jeju National University, Veterinary Medical Research Institute Jeju National University) Corresponding Author