In the present study, a novel ELISA method used recombinant nucleocapsid protein (rNP) as the coating agent. Recombinant Newcastle disease virus (NDV) protein was cloned and expressed in Escherichia coli. Though the rNP-ELISA results were consistent with commercial ELISA results for the NDV-negative sera samples, qualitatively and quantitatively variable (often reduced) results were obtained with NDV-positive sera. Although the rNP-ELISA results for NDV detection were inconclusive, further improvement and standardization of the rNP-ELISA approach, such as using multiple recombinant proteins as the ELISA coating agent and performing comprehensive statistical analyses of combined recombinant protein ELISA, should help counter Newcastle disease outbreaks by improving NDV detection.

• Jihoon Ryu(Research Institute of Veterinary Medicine, Chungnam National University)
• Hyeon Yoon(College of Veterinary Medicine, Chungnam National University)
• Ho-Jin Moon(College of Veterinary Medicine, Chungnam National University)
• Hyun-Jin Shin(Research Institute of Veterinary Medicine, Chungnam National University, College of Veterinary Medicine, Chungnam National University) Corresponding Author