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Dextran Sulfate Sodium으로 유발된 대장염 모델에서 백목이버섯(Tremella fuciformis) 균사체 유래 다당류의 항염증효과 KCI 등재
Anti-Inflammatory Effects of Polysaccharides Isolated from Tremella fuciformis Mycelium on Dextran Sulfate Sodium-Induced Colitis Model
pp.146-155
This study observed the anti-inflammatory effect of the polysaccharide derived from the mycelium of Tremella fuciformis in mice with colitis induced with dextran sulfate sodium (DSS). The experimental groups were normal, DSS, DSS-TFL50, DSS-TFH100, and suflasalazine. Body weights, colon lengths, and organ weights were measured, and the plasma level of pro-inflammatory cytokine and mRNA and protein expression in colon tissue were analyzed. Body weight loss, a symptom of DSS-induced colitis, was suppressed by DSS-TF and the speed of weight recovery proceeded rapidly. In addition, DSS-TF showed a significant inhibitory effect on the decrease of colon length typically caused by colon damage. TNF-α, IL-6 and IL-1β cytokine levels in plasma were reduced in DSS-TF and positive control groups. TNF-α, COX-2 and IL-1β mRNA expression in colon tissue were inhibited in DSS-TF and positive control, and it was significantly different from that of the DSS group. The protein expression of inflammation-related genes (IL-6, TNF-α and COX-2) in the colon tissue was significantly increased by DSS compared to that of the normal group, but by DSS-TFL50, DSS-TFH100 and sulfasalarin decreased. In conclusion, the polysaccharide derived from the mycelium of Tremella fuciformis showed the anti-inflammatory effect on DSS-induced colitis in mice.
서 론
재료 및 방법
1. 실험동물 준비 및 군 구성
2. 대장염 유도 및 시료 투여
3. 실험동물의 체중, 대장길이 및 장기무게측정
4. 마우스 혈청 내 사이토카인 함량 측정
5. RT-qPCR을 이용한 조직 내 mRNA 발현 측정
6. Western blot을 이용한 조직 내 단백질 발현 측정
7. 통계 처리
결과 및 고찰
1. 체중의 변화
2. 장기무게의 변화
3. 대장길이 및 대장무게/길이 비율
4. 마우스 혈청 내 사이토카인 함량 변화
5. RT-qPCR을 이용한 대장 조직 내 mRNA 발현 측정
6. Western blot을 이용한 대장 조직 내 단백질 발현 측정
요약 및 결론
References
