감자뿔나방은 감자에 대한 검역 해충으로 알려져 있다. 본 연구는 전자빔 조사가 감자뿔나방의 발육 및 생식, 그리고 DNA 손상에 미치는 영향을 비교하고 억제선량을 조사하였다. 전자빔을 알(0-12시간 이내), 유충(3령과 5령), 번데기(용화 1일 이내), 그리고 성충(우화 1일 이내)에 선량을 증가시키면서 조사하였다. 전자빔 150 Gy는 알의 부화와 부화된 유충의 용화를 완전히 억제하였다. 조사된 알의 부화율은 19.3%였지만, 성충 우화는 완전히 억제되었다. 3령과 5령 유충에 100 Gy를 조사하였을 때, 성충의 우화와 생식은 완전히 억제되었다. 번데기와 성충에 각각 300 Gy와 400 Gy를 조사하였을 때, F1세대의 부화율이 억제되었다. 전자빔에 대한 감자뿔나방 성충의 DNA 손상 정도를 alkaline comet assay으로 분석하였으며, 전자빔 조사가 선량 의존적으로 감자뿔나방의 DNA 손상 정도를 증가시켰다. 이러한 결과는 감자뿔나방에 대한 식물 검역 처리법으로 전자빔 150 Gy를 권장할 수 있다. 하지만, 감자뿔나방을 방제하기 위해 전자빔을 현장에 적용하기 위해서는 추가적인 연구가 필요할 것으로 사료된다.

The potato tuber moth, Phthorimaea operculella (Zeller) has been known as a quarantine pest of potato. This study investigated inhibition doses of electron beam irradiation (EBM) by comparing their effects on the development and reproduction and DNA damage of the insect pest. Eggs (0-12 h old), larvae (3rd and 5th instar), pupae (less than 1 d old after pupation) and adults (less than 1 d old after emergence) were irradiated with increasing doses of EBM. The EBM with 150 Gy could not completely prevent the hatchability of eggs and pupation of the hatched larvae. The hatchability from the irradiated eggs were 19.3%. However, adult emergence from the irradiated eggs were completely inhibited. When 3rd and 5th instar larvae were irradiated at 100 Gy, the adult emergence from the irradiated larvae and the fecundity of the adults were completely inhibited. When pupae and adults were irradiated at 300 Gy and 400 Gy, respectively, the hatchability of the F1 eggs was completely inhibited. The alkaline comet assay on the level of DNA damage by EBM in P. operculella adults indicates that the EBM increased DNA damage level in a dose-dependent manner, and the damage was repaired in a time-dependent manner. These results may recommend EBM of 150 Gy as a phytosanitary treatment for P. operculella. However further confirmative study is required for the practical application of this EBM dose for P. operculella disinfestation.

ABSTRACT
초 록
재료 및 방법
    실험곤충
    전자빔 조사
    감자뿔나방의 각충태에 대한 전자빔의 효과
    DNA comet assays
    통계분석
결 과
    전자빔의 감수성 조사
    전자빔에 의한 DNA 손상
고 찰
Literature Cited

• 조선란(충북대학교 농업생명환경대학 식물의학과) | Sun-Ran Cho (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University)
• 안현모(충북대학교 농업생명환경대학 식물의학과) | Hyeonmo Ahn (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University)
• 엄태일(충북대학교 농업생명환경대학 식물의학과) | Taeil Eom (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University)
• 경예진(충북대학교 농업생명환경대학 식물의학과) | Yejin Kyung (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University)
• 이승주(충북대학교 농업생명환경대학 식물의학과) | Seung-Ju Lee (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University)
• 김현경(충북대학교 농업생명환경대학 식물의학과) | Hyun Kyung Kim (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University)
• 구현나(충북대학교 농업생명환경대학 식물의학과) | Hyun-Na Koo (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University)
• 김길하(충북대학교 농업생명환경대학 식물의학과) | Gil-Hah Kim (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) Corresponding author