논문 상세보기
pp.109-117
번데기동충하초(Cordyceps militaris(L. ex fr.) Link)의 수소전자공여능 및 항돌연변이원성을 살펴본 결과 부탄을 분획물과 에틸 아세테이트 분획물에서 수소전자공여능이 다른 시료에 비해 2배 이상 높게 나타났다. 항돌연변이원성 실험결과에서는 직접변이원인 MNNG, 4NQO 그리고 간접변이원인 B()P, Trp-P-1에 대해서 유의성 있는 돌연변이 억제효과를 보였다. MNNG(0.4 /plate)의 경우 S. typhimuriu
Cordyceps militaris is a parasitic fungus that has been used as a Chinese medicine for the treatment of fatigue, debility, kidney disease, tuberculosis, asthma and cardiac insufficiency etc. This study was carried out to determine the antioxidative and antimutagenic effects of Cordyceps militaris using DPPH free radical donating method and Ames test, respectively. They were extracted with ethanol and then further fractionated to n-hexane, chloroform, ethyl acetate, butanol and water, stepwise. Among five fractions, the EtOAc and BuOH fractions showed the highest electron donating activities, about 2-fold higher than other fractions. In Ames test, most of the extracts had strong antimutagenic effects against the mutagenesis induced by N-methyl-N'-nitro-N-nitrosoguanidine(MNNG), 4-nitroquinoline-1-oxide(4NQO), benzo()pyrene(B()P) and 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indol (Trp-P-1). The EtOH extracts of C. militaris (200 /plate) showed 62.8%, 74.4% and 67.2% inhibitory effects on the mutagenesis induced by 4NQO, B()P and Trp-P-1, respectively, against TA98 strain, whereas 78.1%, 78.6%, 78.6% and 82.7% inhibition were observed on the mutagenesis induced by MNNG, 4NQO, B()P and Trp-P-1, respectively, against TA100 strain. Especially, the BuOH fraction showed the highest antimutagenic effects against mutation induced by MNNG.
