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pp.295-301
Steaming is a method that has traditionally been used for medicinal plant extraction. This study investigated nitrite oxide production, ferrous ion chelating activity, α-glucosidase, xanthine oxidase, and acetylcholinesterase inhibitory activities of ethanol, acetone and hot-water extracts of Codonopsis lanceolata prepared by steaming seven times. MTT assay showed that each extract was non-toxic up to a concentration of 700 μg/mL confirming that there was no cytotoxicity in all extracts. The α-glucosidase, xanthine oxidase, and acetylcholinesterase inhibitory activities exhibited by the hot-water extract obtained from steaming seven times were higher (83.1%) than the other extracts. Higher production of nitrite oxide and better ferrous chelating activity was recorded with hot-water extract compared to ethanol and acetone extracts. These results indicated that more steaming of Codonopsis lanceolata extracts would be required to validate the possibility of developing antioxidants. Also, further study is needed to determine if the components present in the tested extracts might be useful in the prevention of Alzheimer's disease. These results showed that hot-water extracts may be useful for their antioxidant and the production inhibitory activity of nitrite oxide. It will be helpful in the investigation of the constituent analysis of the steam-processed product of Codonopsis lanceolata.
서 론
재료 및 방법
1. 더덕의 증숙 및 추출물의 제조
2. MTT assay
3. Nitrite oxide 생성 억제 활성 측정
4. 철 이온(Fe2+)에 대한 chelating 효과 측정
5. α-Glucosidase 저해활성 측정
6. Xanthine oxidase 저해활성 측정
7. Acetylcholinesterase 저해 활성 측정
8. 통계 처리
결과 및 고찰
1. 증숙더덕 용매별 추출물의 세포독성
2. Nitrite oxide 생성 억제 활성 및 철 이온(Fe2+)에 대한chelating 효과 측정
3. α-Glucosidase 및 xanthine oxidase 저해활성
4. 아세틸콜린에스테라아제 효소의 활성 측정
요약 및 결론
References
