본 연구에서는 옥수수의 무증자 알콜발효조건을 설정하고자 반응표면분석으로 알콜 발효조건을 모니터링하였다. 그 결과 무증자 발효에 가장 적합한 효모는 S. cerevisiae GRJ이었으며, alcohol content, brix, pH 및 총산에 대하여 회귀 분석한 결과, R2는 각각 0.8852, 0.9202, 0.8806 및 0.9940이였다. 최적 알콜발효 조건은 효소제 함량 0.18%, 가수량 180%(v/w)로 예측되었으며 최적 알콜발효조
This study was carried out to set up alcohol fermentation condition for uncooked corn. Response surface methodology(RSM) was applied to optimize and monitor the alcohol fermentation condition with uncooked corn. The optimal yeast strain for fermentation of uncooked corn was Saccharomyces cerevisiae GRJ. The polynomial equation for alcohol contents, brix, pH and total acidity showed 0.8852, 0.9202, 0.8806 and 0.9940 of R, respectively. The optimal rendition for maximum alcohol contents were 0.18%(w/w) of enzyme concentration and 180%(v/w) of added water content. Predicted values at optimum alcohol fermentation condition agreed with experimental value.