논문 상세보기
pp.338-344
본 연구는 포도 거봉종의 종자와 과피 추출물의 지질과산화 억제 효과와 암세포들에 대한 세포 독성을 살펴보았다. 추출 온도를 달리하여 추출한 종자와 과피의 에탄올 추출물과 추출물에 대한 분획물들을 이용하여 지질과산화물의 억제효과를 측정한 결과, 종자 및 과피 에탄올 추출물 모두 온도변화에 따른 영향은 없는 것으로 나타났으며, 추출물의 농도가 증가할수록 지질과산화물의 감소효과가 증가하였다. 종자 추출물의 경우, 3에서 추출한 에탄올추출물이 20 /
This study was conducted to determine biological activities, such as lipid peroxidation inhibition and cytotoxic effect of ethanol extracts of Black Olympia grape seeds and skins, and of organic solvent fractionated ethanol extracts obtained from grape seeds and skins at different temperatures. Among different extraction temperatures, the ethanol extract of grape seed obtained at 30 had the strongest lipid oxidation inhibition of 60.1%, while the strongest lipid oxidation inhibitory effect of 71.2% was observed in the presence of 20 /㎖ ethylacetate fraction obtained from ethanol extract of grape seeds at 30. The ethanol extract of grape seeds showed more strong lipid oxidation inhibition than that of skin extracts. Similar results were observed in cytotoxic effects. The ethanol extract of grape seeds at 30 exhibited more strong cytotoxicity than that of skin extracts on MCF-7, Hep3B, and A549 cell lines. Among organic solvent fractions extracted from the ethanol extracts of gape seeds and skins, the hexane fraction showed the strongest cytotoxic inhibition of 75.15% and 62.50% on MCF-7 and Hep3B cell in the presence of 1.0 /㎖ respectively. On the other hand, the water fraction showed the strongest cytotoxic inhibition of 65.41% on A549 cell in the presence of 1.0 /㎖. Overall, the ethanol extracts and their fractions of Black Olympia grape seeds showed strong lipid oxidation inhibition and cytotoxicity than those of grape skins.
