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pp.111-120
Agarum clathratum (A. clathratum) is a marine brown algal species that belongs to the Costariaceae family and has antioxidant and anti-microbial properties. However, the anti-inflammatory effects of A. clathratum and the molecular mechanisms involved have not been determined so far. This study aimed to investigate the anti-inflammatory effects of A. clathratum extracts in THP-1 macrophages stimulated by lipopolysaccharide (LPS) derived from Porphyromonas gingivalis. The THP-1 cells were differentiated with 12-O-tetradecanoylphorbol-13-acetate and treated with A. clathratum before LPS stimulation. Cell viability was assessed using the trypan blue exclusion assay. The expression of pro-inflammatory response-associated molecules was evaluated by quantitative real-time polymerase chain reaction and Western blot analysis. A. clathratum treatment inhibited the expression of interleukin-1β in LPS-stimulated THP-1 macrophages without causing any cytotoxicity. The anti-inflammatory effect of A. clathratum resulted in a significant repression of the JNK/c-Jun signaling axis, a key regulator in inflammation responses. This study highlights the possible role of A. clathratum in the inhibition of pro-inflammatory cytokines via suppression of the JNK/c-Jun signaling axis and suggests that A. clathratum could serve as a marine-derived anti-inflammatory agent in periodontitis.
Ⅰ. INTRODUCTION
Ⅱ. MATERIALS AND METHODS
1. Cell culture and chemical treatment
2. Trypan blue exclusion assay
3. Western blot analysis
4. Quantitative (real-time) polymerase chainreaction
5. Statistical analysis
Ⅲ. RESULTS
1. Effect of A. clathratum on the viability ofLPS-stimulated THP-1 cells
2. Effect of A. clathratum on LPS-inducedexpression of IL-1β in THP-1 cells
3. Effects of A. clathratum on LPS-inducedJNK/c-Jun signaling in THP-1 cells
Ⅳ. DISCUSSION
REFERENCES
