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Agarum Clathratum의 JNK/c-Jun 신호기전 조절을 통한 LPS-유도 IL-1β 발현 억제에 관한 연구 KCI 등재

Agarum Clathratum Inhibits LPS-induced IL-1β Expression through the Regulation of the JNK/c-Jun Signaling Pathway

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대한구강악안면병리학회지 (The Korean Journal of Oral and Maxillofacial Pathology)
대한구강악안면병리학회 (Korean Academy Of Oral And Maxillofacial Pathology)
초록

Agarum clathratum (A. clathratum) is a marine brown algal species that belongs to the Costariaceae family and has antioxidant and anti-microbial properties. However, the anti-inflammatory effects of A. clathratum and the molecular mechanisms involved have not been determined so far. This study aimed to investigate the anti-inflammatory effects of A. clathratum extracts in THP-1 macrophages stimulated by lipopolysaccharide (LPS) derived from Porphyromonas gingivalis. The THP-1 cells were differentiated with 12-O-tetradecanoylphorbol-13-acetate and treated with A. clathratum before LPS stimulation. Cell viability was assessed using the trypan blue exclusion assay. The expression of pro-inflammatory response-associated molecules was evaluated by quantitative real-time polymerase chain reaction and Western blot analysis. A. clathratum treatment inhibited the expression of interleukin-1β in LPS-stimulated THP-1 macrophages without causing any cytotoxicity. The anti-inflammatory effect of A. clathratum resulted in a significant repression of the JNK/c-Jun signaling axis, a key regulator in inflammation responses. This study highlights the possible role of A. clathratum in the inhibition of pro-inflammatory cytokines via suppression of the JNK/c-Jun signaling axis and suggests that A. clathratum could serve as a marine-derived anti-inflammatory agent in periodontitis.

목차

Ⅰ. INTRODUCTION
Ⅱ. MATERIALS AND METHODS
    1. Cell culture and chemical treatment
    2. Trypan blue exclusion assay
    3. Western blot analysis
    4. Quantitative (real-time) polymerase chainreaction
    5. Statistical analysis
Ⅲ. RESULTS
    1. Effect of A. clathratum on the viability ofLPS-stimulated THP-1 cells
    2. Effect of A. clathratum on LPS-inducedexpression of IL-1β in THP-1 cells
    3. Effects of A. clathratum on LPS-inducedJNK/c-Jun signaling in THP-1 cells
Ⅳ. DISCUSSION
REFERENCES
저자
  • 한정민(서울대학교 치의학전문대학원 구강병리학교실/치학연구소) | Jung-Min Han (Department of Oral Pathology, School of Dentistry and Dental Research Institute, Seoul National University)
  • 조성대(서울대학교 치의학전문대학원 구강병리학교실/치학연구소) | Sung-Dae Cho (Department of Oral Pathology, School of Dentistry and Dental Research Institute, Seoul National University)
  • 정지훈(서울대학교 치의학전문대학원 구강생리학교실/치학연구소) | Gehoon Chung (Department of Oral Physiology, School of Dentistry and Dental Research Institute, Seoul National University)
  • 최수정(서울대학교 치의학전문대학원 구강병리학교실/치학연구소) | Su-Jung Choi (Department of Oral Pathology, School of Dentistry and Dental Research Institute, Seoul National University)
  • 신지애(서울대학교 치의학전문대학원 구강병리학교실/치학연구소) | Ji-Ae Shin (Department of Oral Pathology, School of Dentistry and Dental Research Institute, Seoul National University)
  • 홍성두(서울대학교 치의학전문대학원 구강병리학교실/치학연구소) | Seong-Doo Hong (Department of Oral Pathology, School of Dentistry and Dental Research Institute, Seoul National University) Correspondence
  • 홍경옥(서울대학교 치의학전문대학원 구강병리학교실/치학연구소) | Kyoung-Ok Hong (Department of Oral Pathology, School of Dentistry and Dental Research Institute, Seoul National University) Correspondence