반응표면분석에 의하여 홍화유 추출조건에 따른 campesterol, stigmasterol, -sitosterol 및 total sterol의 추출 최적화를 모니터링 하였으며, 추출온도(35∼75, X), 추출시간(1∼11시간 X), 중심합성계획으로 추출조건을 최적화하였다. campesterol 최적 추출범위는 추출온도, 추출시간 및 시료의 전처리 가공온도가 각각 59.01, 2.88시간, 75.04 이었다. 그러나 -sitosterol, s

Response surface methodology was used for monitoring and optimizing the extraction conditions of campesterol, stigmasterol, -sitosterol, and total sterols from the safflower seed. The conditions of phytosterol extraction were optimized by using central composite design with the temperature(35∼75, X), the time (1∼11hr, X), and the preheating temperature(60∼100, X) as three variables. The extraction conditions for maximum campesterol content were 59.01(X), 2.88hr(X), and 75.04(X). But stigmasterol, -sitosterol and total sterols were not significantly different under designed extraction condition in this study. Besides, oil was extracted from safflower seed at various conditions and yields were 23.44% at 35 and 20.05% at 80, respectively. Total tocopherol content increased from 0.172% to 0.207% as the extraction temperature increased from 35 to 80. A structured lipids(SL) was synthesized enzymatically by extracted safflower oil and conjugated linoleic acid(CLA). After 24hr reaction, 31.79 mol% CLA was incorporated into the extracted safflower oil.

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