Current seismic fragility functions for buildings were developed by defining damage state threshold based on story drift concerning foreign references and using the capacity spectrum method based on spectral displacement. In this study, insufficient details and dependence on the core location of piloti-type buildings were not reflected in the fragility function because it was developed before the Pohang earthquake. In order to develop an improved one for piloti-type buildings, several types of core were selected, damage state threshold was determined based on the capacity of structural members, and three-dimensional analyses were utilized. As a result, seismic fragility functions based on spectral acceleration were developed for various core locations and different shear strengths of the column stirrup. The fragility of piloti-type buildings significantly varied according to core location, an additional single wall, and whether the contribution of column stirrup was included or not. To estimate fragility more reasonably, it is necessary to prepare the parameters to reflect actual state well.