논문 상세보기
pp.100-105
생열귀나무의 잎을 시료로 생리활성 기능을 검색하기 위해 메탄올, 에탄올, 클로로포름 및 물 추출물을 조제하였다. 얻어진 추출물 대하여 DPPH 자유라디칼 소거법에 의한 항산화성 효과와 S. thphimurium TA98과 TA100을 이용한 항돌연변이원성 효과를 검토하여 다음과 같은 결론을 얻었다. 생열귀나무 잎의 용매별 추출수율은 물 추출물이 24.8%로 가장 높았으며, 그 다음으로는 메탄을 추출물 (21.3%), 에탄올 추출물 (17.0%)
The objective of this study was carried out to investigate biological activities effects of Korean leaf from Rosa davurica Pall. in vitro. They were extracted with methanol, ethanol, chloroform and water. Methods of the antimutagenic used in this experiment were well-known bacterial short term tests which include Ames test and the antigenotoxic used in this experiment was DPPH radical scavenge. All extracts (ethanol, methanol, water) except chloroform extract exhibited 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity with IC50/ of 11.5 /mL, 6.4 /mL, 4.8 /mL. In Ames test, most of extracts had strong antimutagenic effects against the mutagenesis induced by 4-nitroquinoline-1-oxide (4NQO), N-methyl-N’-nitro-N-nitrosoguanidine (MNNG), 3-amino-l,4-dimethyl-5H-pyrido[4,3-b]indol(Trp-P-I) and benzo()pyrene(B()P). The extracts of leaves (200 /plate) showed approximately 60∼80％ inhibitory effect on the mutagenesis induced by 4NQO, Trp-P-1 and B()P against TA98 strain, whereas 60∼80％ inhibition were observed on the mutagenesis induced by MNNG, 4NQO, Trp-P-1 and B()P against TA100 strain. respectively.
