도토리 가루를 알칼리로 침전시키면서 정제하여 도토리 전분을 얻었다. 다시 이 starch를 부탄올 개량법으로 분획하여 도토리 amylose와 amylopectin을 얻었고 Corn starch와 corn amylose, corn amylopectin은 시판되는 시약을 사용하였다. 이 시료들의 분자량 분포도를 관찰하기 위하여 GCP측정을 하였다. GCP측정은 DMSO와 DMF의 용매사용과 표준물질로 pullulan을 사용하여 검정하고 각 시료들의

Purified acorn starch was obtained from alkali precipitation method. Acorn amylose and acorn amylopectin were fractionated from purified acorn starch by butanol improvement method. Gel permeation chromatography (GPC) was used to measure molecular weight distribution of acorn starch, acorn amylose, acorn amylopectin and corn starch, corn amylose, corn amylopectin. GPC measurement diagrams were obtained by each retention time. And then, we used DMSO and DMF as solvent, pullulan as standard material. We calculated the Number-average molar mass (Mn), Weight-average molar mass (Mw) and polydispersity from molecular weight distribution of each sample. As a result of estimating molecular weight using GPC, Mw of amylose has small value than Mw of amylopectin. From this fact, the molecular structural aspects of amylose and amylopectin were predicted and it was in good agrement with the tendency of polydispersity by GPC. The polydispersity of starch had big value than amylose and amylopectin, from this result, it might be known that the range of molecular weight appeared broad by heterogeneous properties of two components. The viscosity of purified acorn starch, amylose, amylopectin seperated from acorn starch, was decreased by increasing the shear rate and raising the temperature exponentially. Acorn starch solutions exhibited pseudoplastic power law fluid behavior.

• 김경이