천연 항균성 peptide인 polylysine을 이용한 항균성 포장재의 개발을 위한 기초자료를 얻기 위하여 polyamide 수지를 기반으로 하는 제조조건을 검토하고 두부를 대상식품으로 하여 병원성 및 부패 관련 미생물에 대한 항균활성을 확인하였다. Polylysine 제조조건에 있어서는 polylysine, polyamide의 농도와 코팅필름의 두께에 따라 항균활성에 큰 차이를 보였으나, 이상의 polylysine, polyamide,
This study were investigated to analyzed an optimum preparation condition of coating film and its antimicrobial activity on pathogenic and deteriorative bacteria to obtain fundamental data for development of active packaging film using antimicrobial peptide, polysine. In the preparation conditions of coating film, antimicrobial activity was depending on the concentration of polylysine and polyamide respectively, and relatively high activity was obtained in the film prepared with more than (w/v) of polylysine, (w/v) polyamide, and more than of film thickness. Concentration of polylysine migrated from coated film to distilled water was reached at the maximum concentration, about 20 ppm after 3 days and in equilibrium after 7 days of soaking in sterilized water. An antimicrobial activity of coated film showed bactericidal effect of about comparing with the control against Bacillus cereus having of initial viable cell numbers, and of about comparing with the control against Klebsiella pneumoniae having of initial viable cell numbers. Consequently, it was revealed that polylysine coating film has a potential of applicable possibility as antimicrobial packaging film.