Strawberry is a stand-out cultivating fruit in Korea. The optimum production of strawberry is highly dependent on growing environment. Smart farm technology, and automatic monitoring and control system maintain a favorable environment for strawberry growth in greenhouses, as well as play an important role to improve production. Moreover, physiological parameters of strawberry plant and it is surrounding environment may allow to give an idea on production of strawberry. Therefore, this study intends to build a machine learning model to predict strawberry’s yield, cultivated in greenhouse. The environmental parameter like as temperature, humidity and CO2 and physiological parameters such as length of leaves, number of flowers and fruits and chlorophyll content of ‘Seolhyang’ (widely growing strawberry cultivar in Korea) were collected from three strawberry greenhouses located in Sacheon of Gyeongsangnam-do during the period of 2019-2020. A predictive model, Lasso regression was designed and validated through 5-fold cross-validation. The current study found that performance of the Lasso regression model is good to predict the number of flowers and fruits, when the MAPE value are 0.511 and 0.488, respectively during the model validation. Overall, the present study demonstrates that using AI based regression model may be convenient for farms and agricultural companies to predict yield of crops with fewer input attributes.