Pest control treatment was carried out using an unmanned automatic pesticide spraying system that can spray pesticides on crops while moving autonomously to control pests in vegetable greenhouse. As a result of examining the control effect on tomato and strawberry on thrips (Frankliniella occidentalis) and greenhouse whitefly (Trialeurodes vaporariorum) pests, 85.6% of yellow flower thrips were found in tomatoes and 87.5% in strawberries, and 81.7% (tomato) and 80.6% (strawberry) of greenhouse whitefly. In addition, the control effect according to the pesticide treatment method showed a control effect of 81.7% of the chemical spraying treatment by manpower and 83.9% of the automatic moving pesticide spraying treatment (F=22.1, p < 0.001). When comparing the control effect between the two treatment sections, there was no significance, but the automatic transfer spraying treatment showed a 2.2% higher effect. On the other hand, as a result of comparing the spraying time of the drug, the automatic unmanned control sprayer had a spraying time of 5 min/10a, which took about 25 min less than the conventional manpower spraying time of 25-30 min/10a. Based on these results, it was judged that the automatic transfer spraying method could be usefully used for efficient pest control in the facility greenhouse during the peak period of development.