In this study, we investigated how satisfaction with on-board training had an effect on career motivation of the trainees. For this purpose, satisfaction for on-board training and career motivation were used as variables. Satisfaction for on-board training were classified into education, environment and operation, and then measurement items were composed. Career motivation consisted of career identity, career insight and career resilience. To establish and test nine hypotheses, a questionnaire survey was conducted by 57 trainees who participated in the Gyeongsang National University on-board sailing training in 2021. To analyze the questionnaire responses, frequency analysis, factor analysis, multiple regression analysis and paired sample t-test were used. As a result of factor analysis, six factors for satisfaction for on-board training were derived. Among the factors, it was analyzed that satisfaction with assignments, the satisfaction with accommodation, and the satisfaction with the adequate condition of facilities and equipment had a significant effect on career motivation. In addition, it was found that career motivation significantly increased through on-board training as a result of comparing the difference between the averages of career motivation before and after on-board training.