The purpose of this study was to analyze the anti-inflammatory effect of Chung-Dae Indigo Pulverata Levis, indigo naturalis) produced during indigo dyeing. As a result of in vitro cytotoxicity experiments using RAW 264.7 cell, Chung-Dae extract did not inhibit cell proliferation in Raw 264.7 cells in the range of 1~32 μg/mL. NO production was significantly reduced when Chung-Dae extracts were treated at concentrations of 2, 8, and 32 μg/mL (p<0.05). The pro-inflammatory cytokines TNF-α, IL-6, IL-1β and IFN-γ significantly decreased when the Chung-Dae extract was treated at concentrations of 2, 8, and 32 μg/mL compared to the LPS group, and similarly, the TNFα and IL-6 mRNA levels also decreased. Additionally, the mRNA level of COX-2 was also suppressed. At the protein expression level, the expression of TNF-α, IL-6, iNOS and COX-2 were observed with LPS and Chung-Dae extract significantly decreased compared to the group treated with only LPS (p<0.05). From the above results, it shows that Chung-Dae extract, a plant-derived compound, inhibits the inflammatory response induced by LPS in RAW 264.7 cells. and in particular, regulates the inflammatory response by inhibiting the expression of pro-inflammatory cytokines and inflammation-related enzymes.

Abstract
서 론
재료 및 방법
    1. 시료 추출물 제조
    2. Raw 264.7 대식세포 배양
    3. MTT assay를 이용한 세포 성장 억제효과
    4. NO 생성능 측정
    5. Enzyme-linked immunosorbent(ELISA) assay 이용한염증관련 사이토카인 측정
    6. RT-qPCR을 이용한 염증관련 유전자의 mRNA level측정
    7. Western blot을 이용한 AGS 세포 내 유전자의 단백질발현 측정
    8. 통계 처리
결과 및 고찰
    1. 청대의 cell viability 측정
    2. 청대의 LPS로 유도된 NO 생성능 억제
    3. 청대추출물이 전염증성 사이토카인 함량에 미치는 영향
    4. 청대추출물이 전염증성 사이토카인 및 염증 관련효소mRNA 발현에 미치는 영향
    5. 청대추출물이 전염증성 사이토카인 및 염증 관련protein 발현에 미치는 영향
요약 및 결론
References

• 장수주(호서대학교 벤처대학원 융합공학과 박사과정 학생, 호서대학교 보건산업연구소 연구원) | Sou Jou Jang (Ph.D. Student, Dept. of Convergence Engineering, Graduate School of Venture, Hoseo University,Researcher, Institute of Health Industry, Hoseo University)
• 강순아(호서대학교 벤처대학원 융합공학과 교수, 호서대학교 보건산업연구소 소장) | Soon Ah Kang (Professor, Dept. of Convergence Engineering, Graduate School of Venture, Hoseo University,Director, Institute of Health Industry, Hoseo University)