본 연구에서는 야관문의 열수 및 ethanol추출조건에 따른 항산화활성의 변화를 비교평가 하고자 하였다. 야관문에 존재하는 항산화물질은 용매분획 과정 중에서 열수추출조건에 비해 ethanol 추출조건에서 항산화 성분이 잘 이행되었고, 열수추출조건에서는 상대적으로 활성이 낮게 나타났다. Silica gel adsorption column chromatography 상의 chloroform-methanol의 용매계에서 각 획분에 대해 DPPH-rad

Hot water and 95%(v/v) ethanol extracts were prepared from dried Lespedeza cuneata seeds and antioxidant compounds were isolated by solvent fractionation, silica gel adslorption chromatography, Sephadex LH-20 column chromatography, and preparative HPLC. Antioxidant activity was measured using DPPH radical scavenging activity. The 80%(v/v)ethanol and ethylacetate fraction of Lespedeza cuneata seed extracts had stronger antioxidant effects than did the n-hexane fraction. The active antioxidant compounds obtained from hot water and 95%(v/v) ethanol extracts may be identical, based on analysis by Sephadex LH-20 column chromatography and preparative HPLC.

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