In the early development of parthenogenetic embryo, cytoplasm and nucleic acid fragmentation may be a cause of lower embryo development. The purpose of this study was to evaluate whether embryonic development and apoptosis factors can be reduced by controlling the in-vitro culture environment by the addition of hormones, pregnancy serum and uterine milk. Our study showed that the activity of Casp-3 increased within the cytoplasm when artificially used hormones to induce the incubation environment, and PCNA's manifestation was low. However, the addition of pregnant serum appeared to lower the Casp-3 activity compared to the other groups. In addition, MMP-9 activity was increased and early embryo development and cytoplasmic fidelity were also increased. Therefore, the results of the present study showed that the use of gestational serum in the development of parthenogenetic embryo inhibit apoptosis and increases cytoplasmic reorganization by natural environmental control in in vitro culture.

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REFERENCES

• Na-Hyeon Jung(Major in the Animal Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University, Anseong 17579, Korea)
• Sang-Hwan Kim(Institute of Genetic Engineering, Hankyong National University, Anseong 17579, Korea)
• Dae-Seung Kim(Major in the Animal Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University, Anseong 17579, Korea)
• Jong-Taek Yoon(Institute of Genetic Engineering, Hankyong National University, Anseong 17579, Korea, Department of Animal Life Science, Hankyong National University, Anseong 17579, Korea) Corresponding author