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In vitro maturation of human pluripotent stem cell-derived cardiomyocyte: A promising approach for cell therapy KCI 등재
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/415518
- DOIhttps://doi.org/10.12750/JARB.37.2.67
pp.67-79
Currently, there is no treatment to reverse or cure heart failure caused by ischemic heart disease and myocardial infarction despite the remarkable advances in modern medicine. In addition, there is a lack of evidence regarding the existence of stem cells involved in the proliferation and regeneration of cardiomyocytes in adult hearts. As an alternative solution to overcome this problem, protocols for differentiating human pluripotent stem cell (hPSC) into cardiomyocyte have been established, which further led to the development of cell therapy in major leading countries in this field. Recently, clinical studies have confirmed the safety of hPSC-derived cardiac progenitor cells (CPCs). Although several institutions have shown progress in their research on cell therapy using hPSC-derived cardiomyocytes, the functions of cardiomyocytes used for transplantation remain to be those of immature cardiomyocytes, which poses a risk of graft-induced arrhythmias in the early stage of transplantation. Over the last decade, research aimed at achieving maturation of immature cardiomyocytes, showing same characteristics as those of mature cardiomyocytes, has been actively conducted using various approaches at leading research institutes worldwide. However, challenges remain in technological development for effective generation of mature cardiomyocytes with the same properties as those present in the adult hearts. Therefore, in this review, we provide an overview of the technological development status for maturation methods of hPSC-derived cardiomyocytes and present a direction for future development of maturation techniques.
INTRODUCTION
An overview of clinical trials for cell therapy usinghPSC-derived CPCs or CMs
1) A clinical study with hESC-derived CPCs
2) A clinical study with hiPSC-derived cardiomyocytesheets
3) A clinical study with hiPSC-derived cardiomyocyte aggregates
Comparison of the characteristics between immaturecardiomyocytes and mature cardiomyocytes
1) Morphology and cellular structure
2) Electrophysiological properties
3) Calcium handling
4) Metabolism
5) Gene expression
Methods of promoting maturation of cardiomyocytes
1) Long-term culture of cardiomyocytes
2) Addition of maturation factor
3) 3-Dimensional (3D) cell culture methods
4) Various other cell co-culture methods
5) Electric stimulation of cardiomyocytes
CONCLUSION REMARKS AND FUTUREPERSPECTIVES
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