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Detection of Sugar Process Contamination Using Dextran Binding hages Produced by Batch Fermentation of Escherichia coli

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  • URLhttps://db.koreascholar.com/Article/Detail/41564
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한국식품저장유통학회 (The Korean Society of Food Preservation)
초록

Sequential passes through columns were used to select phages that displays ligands for dextran (-1,6 linked linear chains) from a phage antibody library. Those phages that bound to the in each iteration were replicated in E. coli. A phage preparation isolated on the third round selection produced 5.4 nephelos turbidity units (NTU) in a dextran specific immunonephelometric assay, a 2.2 fold higher value than the phage preparation from the first round selection. This phage gave normalized intensity (N.I.) in a dip-stick assay against high molecular size dextran (T2000, 30\;{\pm}\;610^4\beta-galactosidase linked assay and polymerase chain reaction.

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