A strain of Alexandrium species was established by isolating cells from Jangmok Bay, Korea. Its morphology and molecular phylogeny based on LSU rRNA gene sequences were examined. In addition, growth responses of this Alexandrium species to changes in temperature, salinity, and nutrient concentrations were investigated. This Alexandrium species from Jangmok Bay had a ventral pore on the 1′, which was morphologically consistent with previously described Alexandrium tamarense and A. catenella. Phylogenetic analyses revealed that this isolate was assigned to A. pacificum (Group IV) within A. tamarense species complex. In growth experiments, relatively high growth rates and cell densities of A. pacificum (Group IV) were observed at 15°C and 20°C. This species also grew under a wide range of salinity. This indicates that this Korean isolate of A. pacificum (Group IV) is a stenothermic and euryhaline species. In growth responses to changes in nutrient levels, enhanced growth rates and cell densities of A. pacificum (Group IV) were observed with additions of nitrate and phosphate. In particular, rapid uptakes of phosphate by A. pacificum (Group IV) were observed in experimental treatments, indicating that the increase in phosphate concentration could stimulate the growth of A. pacificum (Group IV).

Abstract
서 론
재료 및 방법
    1. Alexandrium 종의 분리와 배양
    2. Alexandrium 종의 형태 관찰
    3. Genomic DNA 추출, PCR 증폭 및 염기서열 분석
    4. 계통 분석
    5. 온도, 염분 구배와 영양염 첨가에 따른Alexandrium 종의 성장 반응 실험
결과 및 고찰
    1. Alexandrium pacificum (Group IV)의형태와 계통 분류
    2. 온도 차이에 따른 A. pacificum (Group IV)의성장 반응
    3. 염분 차이에 따른 A. pacificum (Group IV)의성장 반응
    4. 질산염과 인산염 첨가에 따른 A. pacificum(Group IV)의 성장 반응
적 요
사 사
REFERENCES

• 한경하(한국해양과학기술원 해양시료도서관, 한양대학교 환경과학과) | Kyong Ha Han (Library of Marine Samples, Korea Institute of Ocean Science and Technology, Department of Environmental Science, Hanyang University)
• 윤주연(한국해양과학기술원 해양시료도서관) | Joo Yeon Youn (Library of Marine Samples, Korea Institute of Ocean Science and Technology, Geoje 53201, Republic of Korea)
• 곽경윤(한국해양과학기술원 해양시료도서관) | Kyeong Yoon Kwak (Library of Marine Samples, Korea Institute of Ocean Science and Technology, Geoje 53201, Republic of Korea)
• Li Zhun(한국생명공학연구원 생물자원센터)
• 이원철(한양대학교 환경과학과) | Wonchoel Lee (Department of Environmental Science, Hanyang University, Seoul 04763, Republic of Korea)
• 신현호(한국해양과학기술원 해양시료도서관) | Hyeon Ho Shin (Library of Marine Samples, Korea Institute of Ocean Science and Technology, Geoje 53201, Republic of Korea) Corresponding Author