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침수 스트레스에 의한 밭작물의 효소활성 변화 분석 KCI 등재

Analysis of enzyme activity changes caused by flooding stress in upland crops

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  • URLhttps://db.koreascholar.com/Article/Detail/416915
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한국환경생물학회 (Korean Society Of Environmental Biology)
초록

Among various environmental stresses, humid stress lacks mechanisms and biochemical understanding compared to drought, low temperature, and high salt stresses. The aim of this study was to investigate enzyme activity of field crops under humidity stress. Results of this study could be used as basic data for understanding humidity stress and early diagnosis. Growth and enzyme activities of sesame, perilla, red beans, sorghum, and beans as major field crops in Korea when flooded were investigated. It was confirmed that growths of both shoots and roots were retarded. In plants, anaerobic fermentation occurred due to flooding stress, which increased the activity of alcohol dehydrogenase (ADH) compared to the control group. Increases of reactive oxygen species (ROS) were also observed. All flooded plants showed increased peroxidase (POD) activity and lipid peroxidation. Their dyeing strength was darker than that of the control group, even in 3,3´-diaminobenzidine (DAB) staining. Since enzyme activity changes in plants appear relatively faster than changes in phenotype at the ground level, they could be used as biomarkers for early diagnosis of humidity stress in crops.

목차
Abstract
서 론
재료 및 방법
    1. 밭작물의 성장 및 침수 스트레스 조건
    2. 광포화점 측정
    3. ADH 활성 측정
    4. POD 활성 측정
    5. Lipid peroxidation 측정
    6. DAB 염색
    7. 통계분석
결과 및 고찰
    1. 침수 스트레스에 의한 밭작물의 생육도
    2. 침수 스트레스에 의한 밭작물의 생화학적 반응
적 요
사 사
REFERENCES
저자
  • 신주형(경북대학교 농생명융합공학과) | Juhyung Shin (Department of Integrative Biology, Kyungpook National University, Daegu 41566, Republic of Korea)
  • 김병규(경북대학교 농생명융합공학과) | Byeonggyu Kim (Department of Integrative Biology, Kyungpook National University, Daegu 41566, Republic of Korea)
  • 김기환(경북대학교 응용생명과학과) | Kihwan Kim (Department of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea)
  • 강태안(경북대학교 응용생명과학과) | Tae-An Kang (Department of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea)
  • 김원찬(경북대학교 농생명융합공학과, 경북대학교 응 용생명과학과) | Won-Chan Kim (Department of Integrative Biology, Kyungpook National University,2Department of Applied Biosciences, Kyungpook National University) Corresponding Author