Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis (CLA), a chronic contagious disease in small ruminants. The prevalence of CLA has been reported to be >50% in Korean black goats. CLA is difficult to control due to a lack of efficient vaccines and treatment methods. Effective disinfection of the farm environment may be an alternative strategy for reducing the spread of C. pseudotuberculosis. The objective of this study was to evaluate the efficacy of commercial disinfectants against CLA. The six commercial disinfectants, largely composed of sodium dichloroisocyanurate, sodium hypochlorite, potassium monopersulfate triple salt, quaternary ammonium, citric acid, and copper sulfate, were tested against five different genotypes of C. pseudotuberculosis isolated from goat farms in Korea. Efficacy tests were performed in accordance with the disinfectant efficacy test guidelines recommended by the Animal and Plant Quarantine Agency of Korea with slight modifications. All disinfectants except for copper sulfate exhibited >99.99% killing efficacy under hard water conditions following 30 min of incubation, which is the recommended standard treatment time according to guidelines. The minimum bactericidal treatment time was evaluated by employing treatments for durations of 1, 5, and 15 min. The most effective compounds under hard water conditions were sodium dichloroisocyanurate, potassium monopersulfate triple salt, and sodium hypochlorite, exhibiting >99.99% killing efficacy after 1 min of treatment. In the aqueous solution forms, citric acid and the quaternary ammonium compound were the most effective, but required at least 5 min to kill >99.99% of the bacteria. The current study characterizes the killing efficacy of six commercial disinfectant active compounds against C. pseudotuberculosis. Thus, this study provides essential information regarding the efficacy of the disinfectants used to control CLA in goat farms.

ABSTRACT
Materials and Methods
    실험 균주
    소독제 종류
    소독제 효능시험을 위한 희석액 및 배지 준비
    소독제 효능 평가
Results and Discussion
Acknowledgement
국문요약
Conflict of interests
ORCID
References

• 조현우(인제대학교 일반대학원 생명과학과) | Hyeunwoo Cho (Department of Biological Sciences, Inje University, Gimhae, Korea)
• 김연아(인제대학교 일반대학원 생명과학과) | Yeona Kim (Department of Biological Sciences, Inje University, Gimhae, Korea)
• 장범순(인제대학교 일반대학원 생명과학과) | Beomsoon Jang (Department of Biological Sciences, Inje University, Gimhae, Korea)
• 김찬란(농촌진흥청 국립축산과학원 가축유전자원센터) | Chan-Lan Kim (Animal Genetic Resources Research Center, National Institute of Animal Science, Hamyang, Korea)
• 박건택(인제대학교 일반대학원 생명과학과) | Kun Taek Park (Department of Biological Sciences, Inje University, Gimhae, Korea) Corresponding author