Biofilms are complexly structured communities of microorganisms composed of surface-attached microorganisms, where their effects on the host have been controversial. In this study, we investigated the potential biofilm-forming capacity of Lacticaseibacillus rhamnosus LRH020 (DSM25568) by detecting genes known to promote biofilm formation. It was shown that the aggregation substance gene (asa 1) was presented in the LRH020 strain. Therefore, we investigated the phenotypic activities of the gene asa1 via two methods: biofilm formation and autoaggregation activity. It was shown that the strain LRH020 had significantly less ability to form biofilm compared to the positive control strain Enterococcus faecalis ATCC 19433. Furthermore, LRH020 exhibited biofilm-forming activity comparable to Lacticaseibacillus rhamnosus GG (LGG), widely used probiotics. The auto-aggregation activity of LRH020 was also within the safe range similar to that of LGG. In conclusion, this study shows that both biofilmforming and auto-aggregation activities of the LRH020 are comparable to one of the most studied probiotics strains, LGG.

ABSTRACT
Materials and Methods
    사용 균주
    독성 유전자 분석
    미생물막 형성능 확인
    자가응집력 활성 분석
    통계분석
Results and Discussion
    독성 유전자 분석 확인
    미생물막 형성능 확인
    자가응집력 활성 분석
국문요약
Conflict of interests
ORCID
References

• 김혜림((주)씨에이치랩스) | Hye Rim Kim (CH Labs Corp., Chong Kun Dang Health care Labs, Seoul, Korea)
• 서은솔((주)씨에이치랩스, 서울대학교 농업생명과학대학 바이오모듈레이션학과) | Eunsol Seo (CH Labs Corp., Chong Kun Dang Health care Labs, Seoul, Korea / WCU Biomodulation Major, Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul, Korea)
• 서민영(㈜신우코퍼레이션) | Min Yeong Seo (ShinWoo Co., Ltd, Anyang-si, Korea)
• 김병용((주)씨에이치랩스) | Byung-Yong Kim (CH Labs Corp., Chong Kun Dang Health care Labs, Seoul, Korea) Corresponding author