본 연구에서는 미강의 지용성 생리활성 물질인 -oryzanol과 그 모핵인 ferulic acid를 ethanol을 용매로 효율적으로 추출하고자 반응표면분석법을 이용하여 최적 추출 조건을 설정하였다. 중심합성계획법에 따라 추출조건의 독립변수를 시료에 대한 용매비(), 추출 온도(), 추출 시간()으로 하고 이에 따라 영향을 받는 종속변수로 추출 수율(), 총페놀 함량(), 전자공여능(), 미강의 지용성 생리활성물질인 ferulic acid 함량(

We determined the optimum ethanolic conditions for extraction of -oryzanol and other functional components from rice bran, using response surface methodology (RSM). A central composite design was used to investigate the effects of the independent variables of solvent ratio (), extraction temperature (), and extraction time (), on dependent variables including yield (), total phenolic content (), electron-donating activity (), ferulic acid level (), and -oryzanol concentration (). Solvent ratio and extraction temperature were the most important factors in extraction. The maximum yield was at 22.56 mL/g (), 78.19C (), and 522.15 min (), at the saddle point. Total phenolic levels were little affected by solvent ratio or extraction temperature. The maximum concentration of extracted total phenolics was 90.78mg GAE/100 g at 21.26 mL/g, , and 567.97 min. A maximum electron-donating ability of 54.72% was obtained with the parameters 20.20 mL/g,, and 701.87 min, at the highest point. The maximum level of ferulic acid components was 210.47 mg/100g at 5.22 mL/g, , and 575.24 min. In addition, the maximum -oryzanol concentration was 660.39 mg/100g at 5.10 mL/g, , and 587.39 min. The optimum extraction conditions were a solvent ratio of 10.45 mL/g, extraction temperature, and 535 min extraction time. Predicted extraction levels under optimized conditions were in line with experimental values.

• 조인희
• 최용희