Lactic acid bacteria as probiotics are intensively used in human and animal species. These probiotic properties of LABs were variable according to bacterial strain and species. However, there was limited information on probiotic properties of monkey origin LABs. In this study, we investigated the antibacterial activity of monkey and human origin LABs against monkey origin enteric bacteria by the agar disc diffusion test and broth culture inhibition assay. All LABs represented enough tolerance to pepsin (0.3%) and bile acid (pH = 2). To 50% of Clostridium perfringens and 20% of Escherichia coli, monkey origin LABs showed statistically higher antibacterial activity compared to human origin LABs (p < 0.05). Also, distinct antibacterial activity was verified among some bacteria species and strains. Higher antibacterial activity against enteric bacteria except for C. perfringens was verified in Lactobacillus johnsonii strains compared to Lactobacillus reuteri and Lactobacillus salivarius. Statistically different antibacterial activity against C. perfringens was verified among strains within L. reuteri and L. johnsonii. In conclusion, we prove the higher probiotic properties of monkey origin LABs against homogenous enteric bacteria although humans and monkeys were phylogenetically similar species. For non-human primates, homogenous LABs should be used as probiotics, not human origin LABs. Furthermore, it was confirmed among monkey origin LABs, L. johnsonii showed a high antibacterial activity on various enteric pathogenic bacteria and was an appropriate lactic acid strain for inhibiting C. perfringens.

Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

• Phil Yong Kang(College of Veterinary Medicine, Chungbuk National University, Futuristic Animal Resource Center, Korea Research Institute of Bioscience & Biotechnology)
• Kyung-Hyo Do(College of Veterinary Medicine, Chungbuk National University)
• Bon-Sang Koo(National Primate Research Center, Korea Research Institute of Bioscience & Biotechnology, KRIBB School of Bioscience, University of Science and Technology) Corresponding Author
• Wan-Kyu Lee(College of Veterinary Medicine, Chungbuk National University)