The present study was designed to investigate the antiproliferative activity and molecular mechanisms of Bibimbap in HT-29 human colorectal adenocarcinoma cells. Bibimbap extract inhibited the proliferation of HT-29 cells by 50% at a concentration of 10.1±0.17 mg/mL for 48 h. The population of live cells decreased slightly, and the morphology changed with a reduction in cell volume (pyknosis) with Bibimbap. Treatment with 5 mg/mL of Bibimbap resulted in slight cell shrinkage. Furthermore, as the Bibimbap dose increased to 10 mg/mL, these characteristics were more evident, and HT-29 cells exhibited partial detachment by staining with the DNA-binding dye Hoechst 33342. Flow cytometric analysis by Annexin V and PI double staining showed that Bibimbap increased the levels of apoptosis. Analysis of the mechanism of these events showed that Bibimbap-treated cells exhibited a mitochondria-dependent apoptotic pathway through the modulation of caspase-3, caspase-8, caspase-9, and poly-ADP ribose polymerase, as well as Bax and Bcl-2 expression in dose- and time-dependent manners. Consequently, Bibimbap exerts a significant antiproliferative effect on HT-29 human colorectal adenocarcinoma cells.

Abstract
Introduction
Materials and Methods
    1. Preparation of Bibimbap
    2. Chemical reagents
    3. Cell culture and viability assay
    4. Nuclear staining with Hoechst 33342
    5. Apoptosis analysis
    6. RT-PCR
    7. Western blotting analysis
    8. Statistical analysis
Results and Discussion
    1. Inhibitory effects of Bibimbap on FHC and HT-29 cells
    2. Induction of apoptosis on HT-29 cells
    3. Effects of Bibimbap on apoptosis on HT-29 cells
    4. Effects of Bibimbap on the expressions of Bcl-2family and caspase proteins
Conclusion
Acknowledgments
References

• Tae Eun Guon(Graduate Student, Dept. of Food and Nutrition, Duksung Women’s University, Seoul 01369, Korea)
• Ha Sook Chung(Professor, Dept. of Food and Nutrition, Duksung Women’s University, Seoul 01369, Korea) Corresponding Author