The distance between livestock facilities and residential spaces is decreasing. Moreover, livestock odor complaints are increasing due to the large-scale and concentrated livestock breading industry. In order to reduce odor from livestock facilities, bio-curtain that are easy to install and inexpensive are commonly used in Korea. However, there is a lack of basic data on design standards and operation manuals for bio-curtains. The installation density of the bio-curtain material is an important factor that affects the odor reduction rate, increment of the load on the ventilation fans, and the structural stability of the curtain. There are limitations on deriving the design conditions of the bio-curtain by only field experiments targeting invisible air. Therefore, aerodynamic simulation such as CFD (computational fluid dynamics) can be used to obtain quantitative data according to various environmental conditions. Bio-curtain is a porous medium with a complex structure, and it is necessary to derive aerodynamic coefficients to analyze it. In this study, the wind speed and pressure difference according to the design density of the bio-curtain were monitored using the experimental chamber. Using the field results, a pressure resistance curve was created for each flow velocity and installation density. The viscosity and resistance coefficient of the bio-curtain were calculated through the derived resistance curve.