논문 상세보기
pp.374-382
Atlatoxin은 Aspergillus속 곰팡이로부터 생성되며 사람에게 있어서 간독성, 및 간암을 유발하는 잠재력을 가진 대표적 곰팡이 독소이며, 생체에 섭취된 은 여러 가지 대사경로를 거쳐 동물 조직 내에 축적되는 것으로 알려져 있다. 방사선은 수술, 항암약물요법과 더불어 임상 시 중요한 치료방법이나 정상세포에 방사선을 조사하였을 때 반응성이 높은 활성산소와 과산화라디칼()을 생성하여 세포막의 불포화 지방산을 지질 과산화물로 변성시켜 세포 산
Aflatoxin () is a potent hepatotoxic and hepatocarcinogenic mycotoxin in humans. It is also well-known to be accumulated in animal tissues via various metabolic pathways. This study was conducted to determine the effects of vitamin C on the residual in rat sera that were treated with radiation and . Six week-old male Sprague-Dawley rats were randomly divided into five groups: a control group, -treated group, the group treated with and vitamin C, the group treated with X-ray and AFB1, and the group treated with X-ray and with vitamin C. On the first day of the experiment, only one dose of X-rays was exposed to the entire liver at 1,500 cGy. Next, vitamin C was injected at 10 mg/kg body weight via intraperitoneal injection, followed 1 hr later by the administration of 0.4 mg/kg of via intraperitoneal injection. These treatments were then administered every three days over a period of 15 days. On the 16th day of treatments, the animals were sacrificed. The contents of in rat sera were determined via indirect competitive ELISA and HPLC method. In the quantitative analysis of in rat sera via ELISA, ng/mL of was detected in the -treated groups, but the amount more significantly decreased to ng/mL in the groups treated with and vitamin C (p-treated groups. The contents of the rat sera of the groups treated with X-ray and did not significantly decreased with the administration of vitamin C. The content of the rat sera that was analyzed via HPLC showed a tendency similar to that of the content that was analyzed via ELISA. With regard to these data, vitamin C was very effective in reducing residue in rat sera.
