Alfalfa is one of the most useful forage crops worldwide, containing a high level of amino acids that are essential to both human and animal health. However, amino acids and their concentrations may differ between plant parts. Hence, detecting amino acids in different plant parts would be useful in the development of diet supplements. The purpose of this study was to determine the amino acid content in alfalfa leaves, stems, and inflorescences using an amino acid analyzer. Asparagine and glutamic acid were the most abundant amino acids found in stems, leaves, and inflorescences than other amino acids. All parts of alfalfa had low concentrations of cysteine and methionine. All amino acids except asparagine were present in the highest concentration in leaves followed by inflorescences. Leaf had a rich amino acid content, namely asparagine, glutamic acid, leucine, proline, and lysine. However, the stem had a lower amino acid composition than the leaf or inflorescence. Overall, the data showed determining the amino acid content of forages provides a good approach to making animal feed with essential and specific amino acids and preventing excessive inclusion of amino acids.

ABSTRACT
Ⅰ. INTRODUCTION
Ⅱ. MATERIALS AND METHODS
    1. Cultivation of alfalfa
    2. Determination of Amino acids content
    3. Statistical Analysis
Ⅲ. RESULTS AND DISCUSSION
Ⅳ. CONCLUSIONS
Ⅴ. ACKNOWLEDGMENTS
Ⅵ. REFERENCES

• Karnan Muthusamy(Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration)
• Soundharrajan Ilavenil(Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration)
• Jeong Sung Jung(Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration)
• Bae Hun Lee(Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration)
• Cheol Hwan Nam(Livestock Institute, Jeollanamdo Agricultural Research and Extension Services)
• Hyung Soo Park(Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration)
• Ki Choon Choi(Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration) Corresponding author