Bis(2-ethylhexyl) phthalate (DEHP) is one of the plasticizers used in the polyvinyl chloride (PVC) industry. It is known to be easily released into the environment. In this study, we investigated effects of DEHP on growth, metabolic pathway, and virulence gene expression in soil-borne bacterial plant pathogen, Pectobacterium carotovorum SCC1 using in vitro assays. As a result, DEHP at 20 μg mL-1 did not affect the growth, cell membrane permeability, or ATPase activity of P. carotovorum SCC1. However, it decreased succinyl-CoA synthase (SCS) activity in the tricarboxylic acid (TCA) cycle. Relative expression levels of virulence genes encoding pectate lyase and pectin were differentially influenced by DEHP treatment. These results suggest that biological characteristics of P. carotovorum might be influenced by DEHP in soil.

Abstract
서 론
재료 및 방법
    1. 세균 배양과 개체군 평가
    2. TCA cycle
    3. 막 투과성과 ATPase 활성
    4. 병원성 관련 유전자 발현
    5. 통계분석
결과 및 고찰
적 요
사 사
REFERENCES

• 김유리(농촌진흥청 국 립농업과학원 농업미생물과) | Yu-Ri Kim (Division of Agricultural Microbiology, National Institute of Agricultural Sciences)
• 김상태(농촌진흥청 국 립농업과학원 농업미생물과) | Sang Tae Kim (Division of Agricultural Microbiology, National Institute of Agricultural Sciences)
• 상미경(농촌진흥청 국 립농업과학원 농업미생물과) | Mee Kyung Sang (Division of Agricultural Microbiology, National Institute of Agricultural Sciences) Corresponding author