포도씨에 함유된 항산화 및 아질산염소거성분에 대한 마이크로웨이브 최적 추출조건을 예측하고자, 추출조건(microwave power 0~120 W, 에탄올 농도 0~100%, 추출시간 1~5 min)을 독립변수로 하고 조건별 추출물의 총페놀 함량, 항산화능 및 아질산염소거능을 종속변수로 하여 회귀 분석함으로써 최적 추출조건을 예측하였다. 도출된 회귀식의 는 0.8024-0.9498 범위로 나타났다. 추출물 기능성분들의 최대 예측값과 최적 추출조건은

Response surface methodology (RSM) was used for the microwave-assisted extraction (MAE) of the effective components of grape seed, such as its antioxidative and nitrite-scavenging abilities. Microwave power (2,450 MHz, 0-160W), ethanol concentration (0-100%), and MAE time (1-5 min) were used as independent variables (Xi) for the central composite design to yield 16 different MAE conditions. The optimum MAE conditions were predicted for the dependent variables of the extracts, such as the total phenolic content () antioxidative ability (), and nitrite-scavenging ability (), depending on different microwave powers, ethanol concentrations, and MAE times. The determination coefficients () of the regression equations for the dependent variables ranged from 0.8024 to 0.9498. The maximal values of each dependent variable were predicted at different MAE conditions, as follows: 3.19% total phenolic content at 142.32W, 44.30% ethanol, and 4.36 min, and 1.22 antioxidative ability at 84.44W, 56.60% ethanol, and 3.28 min. More than 99.5% nitrite-scavenging ability was predicted at pH 1.2-3.0, 30.80-106.58W, 49.32-55.18% ethanol, and 3.72-4.58min, respectively. The results indicated that the total phenolic content and anti oxidative ability showed a higher correlation with each other in that they were more influenced by microwave power than by the other variables, while the nitrite-scavenging ability was largely influenced by the ethanol concentration.

• 이은진
• 김정숙
• 김현구
• 권중호