Under constant environmental pollution, the incidence of Atopic Dermatitis (AD) caused by air pollutants and allergens has increased. AD is an allergy inflammatory skin disease characterized by pruritus, eczema, and skin dryness. In herbal medicine, Anemarrhena asphodeloides (Anemarrhenae Rhizoma; AR) has been utilized to treat Alzheimer’s disease, osteoporosis, hypertension, and inflammation. The purpose of study evaluated the effect of AR in a mouse model of 2,4-dinitrochlorobenzene (DNCB)-induced AD-like skin lesions. After acclimatization for 5 days, the mice (6-week-old, male Balb/c) were divided into five groups (n=6/group): NC (normal control), DNCB (control), Dex (5 mg·kg-1, p.o.), AR100 (100 mg·kg-1, p.o.), and AR300 (300 mg·kg-1, p.o.). On days 1 and 3, 1% DNCB was applied to the skin and ears. After 4 days, 0.5% DNCB was applied once every 2 days for 2 weeks. Then, skin and ears eczema area and severity index (EASI); skin nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and prostaglandin E2 (PGE2) levels; and plasma immunoglobulin E (IgE) levels were examined. The AR groups showed lower EASI, skin and ear thickness, mast cell count, and IgE levels than the control groups. Moreover, AR reduced iNOS, COX-2, and PGE2 levels. Therefore, AR possesses anti-inflammatory properties and can improve skin damage, indicating its therapeutic potential against AD.

Abstract
1. 서 론
2. 재료 및 방법
    2.1. 재료 및 시약
    2.2. 동물 모델
    2.3. 아토피 피부염 중증도 평가 및 조직면역학적 분석
    2.4. 혈청 내 IgE 발현량 측정
    2.5. 피부 조직 내 iNOS, COX-2, PGE2 발현량 측정
    2.6. 통계처리
3. 결과 및 고찰
    3.1. 실험동물의 체중 변화 및 피부 변화
    3.2. 조직면역학적 변화
    3.3. 혈청 내 IgE 발현량 분석
    3.4. 피부 조직 내 iNOS, COX-2, PGE2 발현량 변화
4. 결 론
감사의 글
REFERENCE

• 장유미(한국한의학연구원 한약자원연구센터, 대구한의대학교 한의학과) | Yumi Jang (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, College of Korean Medicine, Daegu Haany University)
• 김용웅(대구한의대학교 제약공학과) | Yong-Ung Kim (Department of Pharmaceutical Engineering, College of Biomedical Science, Daegu Haany University, Gyeongsan 38610, Korea)
• 김미려(대구한의대학교 한의예과) | Mi Ryeo Kim (Department of Herbal Pharmacology, College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Korea)
• 임혜선(한국한의학연구원 한약자원연구센터) | Hye-Sun Lim (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, Naju 58245, Korea)
• 박건혁(한국한의학연구원 한약자원연구센터, 과학기술연합대학원대학교(UST) 한의융합의학전공) | Gunhyuk Park (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, University of Science & Technology (UST), Korean Convergence Medicine Major, Campus of Korea Institute of Oriental Medicine) Corresponding author