To investigate the anti-inflammatory activity of submerged culture using Cordyceps militaris mycelium, culture-including mycelia was extracted and lyophilized into postbiotics (hot-water extract; CM-HW). HW was fractionated into crude polysaccharide (CM-CP) by ethanol precipitation, and CM-CP was further dialyzed into CM-DCP by dialysis with running water using 12~14 kDa dialysis tube. When the cytotoxicity of subfractions against cells was assessed, no subfraction had a cytotoxic impact that was substantially different from the control groups. In an inflammatory model using LPS-stimulated RAW 264.7 cells, CM-DCP significantly decreased IL-6 and MCP-1 production levels compared to the LPS-control group. CM-DCP also inhibited IL-6 and IL-8 secretion in HaCaT keratinocytes stimulated with TNF-α and IFN-γ. In the meanwhile, the neutral sugar content and mannose ratio of anti-inflammatory CM-DCP were higher than the other fractions, and CM-DCP contained β-1,3/1,6-glucan of 216.1 mg/g. High pressure size exclusion chromatography revealed that CM-DCP contained molecules with a molecular weight range of 5.6 to 144.0 kDa. In conclusion, postbiotics of C. militaris mycelium significantly promoted anti-inflammatory activity, suggesting that neutral polysaccharides including Glc and Man contribute to the anti-inflammation in RAW 264.7 or HaCaT cells.

Abstract
서 론
재료 및 방법
    1. 동충하초 균사체 액체발효 및 포스트바이오틱스 제조및 분획
    2. 세포주 및 배양조건
    3. RAW 264.7 대식세포를 이용한 항염증 활성
    4. HaCaT 세포를 통한 항염증 활성
    5. 분자량 분포
    6. 구성분 및 구성당 분석
    7. β-1,3/1,6-glucan 함량 분석
    8. 통계처리
결과 및 고찰
    1. 동충하초 균사체 액체발효물 유래 포스트바이오틱스분획획분의 세포독성
    2. RAW 264.7 세포주를 이용한 염증 억제
    3. HaCaT 세포를 이용한 피부염 억제 활성
    4. 분자량 분석
    5. 구성분 및 구성당 분석
    6. β-1,3/1,6-glucan 분석
요약 및 결론
감사의 글
References

• 김연숙(한국교통대학교 식품영양학전공 대학원생) | Yeon Suk Kim (Graduate School Student, Major in Food and Nutrition, Korea National University of Transportation, Jeungpyeong 27909, Korea)
• 신현영(고려대학교 대학원 의생명융합과학과 러닝헬스시스템융합전공 대학원생) | Hyun Young Shin (Graduate School Student, Transdisciplinary Major in Learning Health System, Dept. of Integrated Biomedical & Life Science, Korea University, Seoul 02841, Korea)
• 김훈(중앙대학교 생명공학대학 연구교수) | Hoon Kim (Research Professor, College of Biotechnology & Natural Resources, Chung-Ang University, Anseong 17546, Korea)
• 정은진(고려대학교 대학원 의생명융합과학과 러닝헬스시스템융합전공 대학원생) | Eun-Jin Jeong (Graduate School Student, Transdisciplinary Major in Learning Health System, Dept. of Integrated Biomedical & Life Science, Korea University, Seoul 02841, Korea)
• 김현경(숙명여자대학교 식품영양학과 대학원생) | Hyun-Gyeong Kim (Graduate School Student, Dept. of Food & Nutrition, Sookmyung Women’s University, Seoul 04310, Korea)
• 서민근(고려대학교 대학원 의생명융합과학과 러닝헬스시스템융합전공 대학원생, (주)네오크레마 연구개발부 주임연구원) | Min Geun Suh (Graduate School Student, Transdisciplinary Major in Learning Health System, Dept. of Integrated Biomedical & Life Science, Korea University, Senior Researcher, Dept. R & D, Neo-Cremar Corporation, Ltd.)
• 서형주(고려대학교 대학원 의생명융합과학과 러닝헬스시스템융합전공 교수) | Hyung Joo Suh (Professor, Transdisciplinary Major in Learning Health System, Dept. of Integrated Biomedical & Life Science, Korea University, Seoul 02841, Korea)
• 유광원(한국교통대학교 식품영양학전공 교수) | Kwang-Won Yu (Professor, Major in Food & Nutrition, Korea National University of Transportation, Jeungpyeong 27909, Korea) Corresponding author