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Establishment of withdrawal time and analysis of chlorpheniramine maleate residue in milk of intramuscularly administered dairy cows KCI 등재

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예방수의학회지 (Journal of Preventive Veterinary Medicine)
한국예방수의학회(구 한국수의공중보건학회) (The Korean Society of Preventive Veterinary Medicine)
초록

This study investigated chlorpheniramine maleate (CPM) residues in milk of intramuscularly dosed dairy cows and established the withdrawal time (WT) of CPM in milk. Sixteen healthy Holstein cows were injected with 10 (CPM-1) and 20 mL (CPM-2) of the drug containing 4 mg/mL of CPM, respectively. After administration of CPM, milk samples were collected from all cows at 12 hour intervals for 5 days. CPM residue concentrations in milk were determined using LC-MS/MS. The correlation coefficient of the calibration curve was 0.9956, and the limits of detection and quantification (LOQ) were 0.6 and 1.0 μg/kg, respectively. Recoveries ranged between 98.5-115.0%, and coefficient of variations were less than 10.96%. After treatment, CPM in CPM-1 and CPM-2 was detected below the LOQ in all milk samples at 12 hours. According to the European Medicines Agency’s guideline on determination of withdrawal periods for milk, withdrawal periods of both CPM-1 and CPM-2 in milk were established to 12 hours. In conclusion, the developed analytical method is sensitive and reliable for detection of CPM in milk, and the estimated WT of CPM in bovine milk will contribute to ensuring the safety of milk.

목차
Abstract
서 론
재료 및 방법
    시약 및 재료
    실험동물
    시험약제 투여 및 시료채취
    표준원액 및 표준용액의 조제
    시료 전처리
    기기분석조건
    분석법 검증
    우유 중 CPM의 잔류휴약기간 설정
결 과
    CPM의 크로마토그램
    검량곡선, 검출한계 및 정량한계
    회수율과 정밀성
    우유 중 CPM 잔류 분석
    우유 중 CPM 잔류휴약기간 설정
고 찰
감사의 글
REFERENCES
저자
  • Chun-Nam Cha(Engineering Research Institute, Department of Industrial Systems Engineering, Gyeongsang National University)
  • Jonghyup Kim(Department of Anaesthesiology and Pain Medicine, Busan Paik Hospital, College of Medicine, Inje Unversity)
  • Suk Kim(Institue of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
  • Ki-Rim Kim(Department of Dental Hygiene, Kyungpook National University)
  • Seunghee Ki(Department of Anaesthesiology and Pain Medicine, Busan Paik Hospital, College of Medicine, Inje Unversity) Corresponding Author
  • Hu-Jang Lee(Institue of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) Corresponding Author