This study aimed to isolate and identify the new strains which can be utilized in fermentation process for the production of functional materials. Isolation of the new microorganism in wood vinegar, natural liquid material generated from the carbonization of various trees was studied. Marine agar medium was used for the isolation of halo-tolerant bacteria. Using a sterilized toothpick, transfer same shape colonies to agar plates and continuous cultivation of colonies at 37℃ for several days, 2 colonies were isolated. Through the 16S-based ID service, isolated strains were identified as Cytobacillus species. Verifying the industrial values of the two isolated strains, the productivities of various enzymes such as amylase, lipase, and protease were confirmed. As a result, isolated 2 strains showed amylase and protease activities which means the possibility of applying to biological processes in the food and cosmetic industries.

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References

• 조석철(서원대학교 식품공학과) | Seok-Cheol Cho (Department of Food Science & Engineering, Seowon University, Cheongju 28674, Korea)
• 이용직(서원대학교 바이오코스메틱학과) | Yong-Jik Lee (Department of Bio-Cosmetics, Seowon University, Cheongju 28674, Korea) Corresponding author