To isolate aerobically and identify the diversity of halophilic bacteria in the soil around two ports, Daepopogu and Hwasun Port, on Jeju island, a total 46 halophilic bacteria strains were isolated and phylogenetically analysed. The isolated strains were divided into 3 phyla, 8 families, 16 genera and 23 species. The main taxa was the Bacilli class, which included 50.0% of the strains with 3 families, 10 genera and 15 species of Bacillaceae, Exiguobacterium_f and Planococcaceae. The second taxa was the Gammaproteobacteria class, which included 45.7% of the strains with 4 families, 5 genera and 7 species of Aeromonadaceae, Halomonadaceae, Marinobacteraceae and Vibrionaceae. The isolated strains were tested for hydrolytic enzymes, amylase, lipase and protease activity, and 31 strains showed activity of at least one enzyme. Furthermore, auxin activity was determined in 7 strains. This study showed that the isolated strains have possible applications in the food and agricultural industries and have importance as a genetic resource in Korea.

본 연구는 국내외 해양유래 발효산물 시료로부터 분리한 호염성 미생물들의 다양성 및 특성에 관하여 조사하였다. 호염성 미생물의 순수 분리를 위하여 marine agar 배지를 사용하였으며 25, 37, 50oC에서 호기적으로 배양하였다. 순수 분리 후, 86균주를 분리하였으며 16S rRNA 염기서열 분석 결과를 바탕으로 계통학적 분석을 실시한 결과, 3문, 7과, 9속, 24종으로 구성되어 있는 것을 확인하였다. 특히 Firmicutes문 Bacilli강은 84.9%의 분포를 나타내었으며 4과, 6속, 19종으로 Bacillaceae, Planococcaceae, Staphylococcaceae와 Enterococcaceae로 분포하는 것을 확인하였다. 그리고 분리한 균주들이 amylase, lipase, cellulase, protease 같은 산업적으로 유용한 효소를 생산하는지 확인하기 위하여 효소 활성 평가를 실시하였으며, 55 균주가 최소 한 종류 이상의 효소 활성을 가지고 있는 것을 확인하였다. 또한 옥신 생산능을 가지는 균주도 2 균주가 확인되었으며 이는 본 연구를 통하여 분리한 미생물들의 산업적 활용 가능성을 나타내었다. 그러므로 이번 연구는 국내 유전자원 확보 및 시료의 호염성 미생물의 다양성과 특성에 관한 과학적 지식 확장에 도움이 될 것으로 생각된다.

The diversity and characterization of microorganisms isolated from the soil around several ranches in Korea were confirmed in this study. To isolate halophilic microorganisms, the marine agar medium was basically used and cultivated at 37℃ for several days. After single colony isolation, a total of 116 pure colonies were isolated and phylogenetic analysis was carried out based on the result of 16S rDNA sequencing, indicating that isolated strains were divided into 4 phyla, 23 families, 30 genera and 51 species. To confirm whether isolated strain can be a candidate for the fermentation of diverse food ingredients, amylase, lipase, and protease enzyme assays were performed individually, showing that 92 strains possessed at least one enzyme activity. Especially 4 strains, identified to Jonesia quinghaiensis (isolate name: JSF 19-2), Halomonas alkaliantarctica (isolate name: JSF 21), Bacillus velezensis (isolate name: NWFY-36), and Staphylococcus capitis subsp. urealyticus (isolate name: MSY-5), showed all enzyme activity tested. Moreover, 17 strains showed the ability for auxin production. This result indicated that isolated strains have shown the possibility of the application for the food and feed industries. Therefore, this study has contributed to securing domestic biological resources and the improvement of hydrolytic enzyme activity by using isolated strains.

This research confirmed the diversity and characterization of halophilic microorganisms isolated from the various solar salterns, collected on the inside and outside of the country. To isolate strains, the marine agar medium was basically used and cultivated at 37oC for several days aerobically. After single colony isolation, a totally of 230 pure colonies were isolated and phylogenetic analysis was carried out based on the result of 16S rDNA sequencing, indicating that isolated strains were divided into 4 phyla, 12 families, 27 genera and 64 species. Firmicutes phylum, the main phyletic group, comprised 89.6% with 3 families, 17 genera and 52 species of Bacillaceae, Staphylococcaceae and Carnobacteriaceae. To confirm whether isolated strain can produce industrially useful enzyme or not, amylase, lipase, and protease enzyme assays were performed individually, showing that 177 strains possessed at least one enzyme activity. Especially 17 strains showed all enzyme activity tested. This result indicated that isolated strains have shown the possibility of the industrial application. Therefore, this study has contributed to securing domestic genetic resources and the expansion of scientific knowledge of the halophilic microorganisms community in solar salterns.

The malodor control law enacted in 2005, regulated malodor substances ansmg from factory or service industry. In this work, an attempt is made to understand the actual conditions of malodor occurrence for types of industry and to settle trouble for a odor complaint. The odor concentration unit (o.u/㎥) of each major odor emitting industry was actualized by the indirect olfactory method including air dilution sensory test method in Korea. The industry that malodor complaint originate mainly were Chemical manufacturing industry, Waste disposal facilities and Livestock farming. As the results, Livestock farming were measured from 3 o.u/㎥ to 30 o.u/㎥ in the source boundary site, Waste disposal facilities were measured from 300 o.u/㎥ to 669 o.u/㎥ in the stack and Chemical manufacturing industry was analyzed from 300 o.u/㎥ to 1442 o.u/㎥ in the stack. Sampling of the source boundary site was demand the reform because malodor state at the field not expressed properly. In oder to solve such problem, propose total odor emission rate (T.O.E.R) introduction.

This study was performed to investigate the characteristics of indoor air quality (IAQ) in elementary and middle schools in Gyeongbuk area from July to November 2006 . The measurements of indoor air pollutants were made to cover such components as PM-10, CO2, CO, NO2, O3, HCHO, TVOC, TBC, and Radon from school classrooms. The results of this study can be summarized as follows: all of them were less than IAQ standards of Korea. The mean concentration were measured by 43.0 ㎍/㎥ (PM-10), 745 ppm (CO2), 56.1 ㎍/㎥ (HCHO), 350 CFU/㎥ (TBC), 0.026 ppm (O3), 0.6 ppm (CO), and 59.2 ㎍/㎥ (TVOC). Radon was not detected in all surveyed classrooms. The I/O ratio of PM-10 was 0.8∼1.4, while that for HCHO was 5.7∼9.0. Therefore, it was concluded that the indoor pollution of formaldehyde was very serious in classroom.

This research focused on the isolation and characterization of the new strains which can be used as microbial resources for auxin production in the agriculture industry. For the isolation of the new microorganism in the gut of Larimichthys polyactis, a marine agar medium was used and 3 colonies were isolated. Through the 16S-based ID service, isolated strains were identified as 1 strain of Niallia circulans and 3 strains of Proteus mirabilis. Verifying the agriculture industrial values of these isolated strains, the productivity of auxin and activity of various enzymes such as amylase, lipase, and protease were confirmed. As a result, isolated 3 strains showed auxin activity and only protease activities which means the possibility of applying them to the production of effective microorganisms in the agriculture industry.

Screening of Bacillus subtilis strains capable of producing extracellular hydrolases, including lipolytic enzyme was carried out. Within the scope of this study, total 61 strains were isolated from various domestic specific environmental samples such as seawater, soil, Jeju ranch, hot spring, salt farm, and fermented food and identified as Bacillus subtilis based on 16S rRNA sequences. Among the isolates, 4 strains had extracellular lipase activity. The growth profile of the strains revealed that 4 strains showed well growth at 50℃, 3 strains at 55℃, and pH 7.0. All strains could tolerate salinity up to 5%(w/v), 3 strains up to 10%(w/v), and only one strain showed growth at 15%(w/v). Additionally, amylolytic and proteolytic activities were detected in these strains. The highest lipolytic, amylolytic, and proteolytic activity was detected in Bacillus subtilis YS-YR 5A. These results demonstrate the potential application of extracellular hydrolase-producing Bacillus subtilis strains, especially strain YS-YR 5A, as fermentation starter to enhance the functionality and multiplication of functional natural products in the food/medicine/cosmetics/bio industries.

This study aimed to isolate and identify the new strains which can be utilized in fermentation process for the production of functional materials. Isolation of the new microorganism in wood vinegar, natural liquid material generated from the carbonization of various trees was studied. Marine agar medium was used for the isolation of halo-tolerant bacteria. Using a sterilized toothpick, transfer same shape colonies to agar plates and continuous cultivation of colonies at 37℃ for several days, 2 colonies were isolated. Through the 16S-based ID service, isolated strains were identified as Cytobacillus species. Verifying the industrial values of the two isolated strains, the productivities of various enzymes such as amylase, lipase, and protease were confirmed. As a result, isolated 2 strains showed amylase and protease activities which means the possibility of applying to biological processes in the food and cosmetic industries.

수박에서 종자크기의 유전분석을 위해 종자크기가 다른 6계통을 양친으로 한 교배집단을 조사하였다. 전체 6계통 중 3계통은 수집 계통으로 giant seed(GS)인 'PI525088' big size(BS)인 'Charleston Gray' 그리고 medium seed(NS)인 'NT'를 사용하였으며, 다른 3계통은 보통 크기와 가장 작은 크기 계통간('NT'× 수식 이미지'TDR') 교잡 및 여교잡으로부터 육성되어 종자크기만 상이한 nea

수박에서 종자크기가 다른 각 계통들간의 종자특성과 종자 크기의 변화에 따른 과특성을 살펴보기 위해 수박 유전자원 353 점을 대상으로 종자의 크기를 조사하고 6가지 대표적인 종자크기 형으로 분류하였으며 종자크기가 큰 순서부터 giant seed(GS), big seed(BS), medium size(NS), small size(SS), micro seed(MS), tomato seed(TS)라고 명명하였다. 종자크기가 작아질수록 종장, 종경이 작아졌고,