This research focused on the isolation and characterization of the new strains which can be used as microbial resources for auxin production in the agriculture industry. For the isolation of the new microorganism in the gut of Larimichthys polyactis, a marine agar medium was used and 3 colonies were isolated. Through the 16S-based ID service, isolated strains were identified as 1 strain of Niallia circulans and 3 strains of Proteus mirabilis. Verifying the agriculture industrial values of these isolated strains, the productivity of auxin and activity of various enzymes such as amylase, lipase, and protease were confirmed. As a result, isolated 3 strains showed auxin activity and only protease activities which means the possibility of applying them to the production of effective microorganisms in the agriculture industry.

서 론
재료 및 방법
    세균 분리 및 배양
    16S rDNA 염기서열의 계통학적 분석
    옥신(Auxin) 생성능 분석
    세포외 분해 효소 생산능 분석
결과 및 고찰
    균주 분리 및 배양
    16S rDNA 염기서열 분석을 통한 분리 균주 동정
    옥신 생산능과 세포외 분해효소 활성능 분석
요 약
사 사

• 김명기(서원대학교 식품공학과) | Myong Ki Kim (Department of Food Science & Engineering, Seowon University, Cheongju 28674, Korea)
• 이상재(신라대학교 바이오식품공학과) | Sang-Jae Lee (Department of Food Biotechnology, Silla University, Busan 46958, Korea) Corresponding author
• 이용직(서원대학교 바이오코스메틱학과) | Yong-Jik Lee (Department of Bio-Cosmetics, Seowon University, Cheongju 28674, Korea) Corresponding author