The blocked N-terminus and N-terminal sequence ol soybean β-amylase were determined by analyzing the acidic peptides derived on peptic digestion of the enzyme. The acidic peptides were separated from the digest on a Dowex 50×2 column(1×5㎝) and purified by reversed phase-high performance liquid chromatography(RP-HPLC). The major acidic peptide, PEP-1, was a heptapeptide. The N-terminal 7 amino acid sequence of soybean β-amylase was deduced to be acetyl-Ala-Thr-Ser-Asp-Ser-Asn-Met- from the results of sequence analysis of PEP-1 and amino acid analysis of other acidic peptides.

• 김하경 | Ha Kyung Kim
• 황성연 | Seong Yun Hwang
• 윤수봉 | Soo Bong Yoon
• 천덕상 | Dug Sang Chun
• 공석길 | Suk Kil Kong
• 강근옥 | Kun Og Kang