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대두 β - Amylase 의 N - 말단 아미노산 배열 KCI 등재

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한국식품영양학회지 (The Korean Journal of Food And Nutrition)
한국식품영양학회 (The Korean Society of Food and Nutrition)
초록

The blocked N-terminus and N-terminal sequence ol soybean β-amylase were determined by analyzing the acidic peptides derived on peptic digestion of the enzyme. The acidic peptides were separated from the digest on a Dowex 50×2 column(1×5㎝) and purified by reversed phase-high performance liquid chromatography(RP-HPLC). The major acidic peptide, PEP-1, was a heptapeptide. The N-terminal 7 amino acid sequence of soybean β-amylase was deduced to be acetyl-Ala-Thr-Ser-Asp-Ser-Asn-Met- from the results of sequence analysis of PEP-1 and amino acid analysis of other acidic peptides.

저자
  • 김하경 | Ha Kyung Kim
  • 황성연 | Seong Yun Hwang
  • 윤수봉 | Soo Bong Yoon
  • 천덕상 | Dug Sang Chun
  • 공석길 | Suk Kil Kong
  • 강근옥 | Kun Og Kang