토양에서 분리한 Streptomyces SP. 균주에서 분비되는 chitobiase의 효소학적 성질을 검토하였다. 최적반응조건은 pH 5.5, 40℃이었으며, 효소의 안정성은 pH 4.0∼5.5, 40℃에서 40분간 이었다. 45℃에서 1시간 열처리하였더니 효소의 활성은 거의 실활되었다. Mn^2+, Mg^2+이온에 의해 활성이 약간 증가하였으며 Fe^2+이온은 저해시켰으며, Km값과 활성화 에너지는 각각 1.5072mM, 8.314㎉/㏖이었다.

Streptomyces sp. YB-88-20 was isolated from soil and the properties of chitobiase were investigated. The optimal reaction condition for the enzyme was pH 5.5 and 40℃, and was stable in the range of pH 4.0 to 5.5 and temperature at 40℃, and 40min, respectively. The enzyme was inactivated by heating at 45℃ for 1hr. The enzyme was slightly activated by Mn^2+, Mg^2+, but inhibited by Fe^2+. Km and activation energy was 1.5072mM and 8.314㎉/㏖.

• 소명환 | Myung Hwan So
• 박상희 | Sang Hee Park
• 조신호 | Shin Ho Cho
• 황한준 | Han Joon Hwang
• 성낙주 | Nak Ju Sung