논문 상세보기
pp.268-275
Actinoplanes missouriensis KCTC 1780를 packed bed bioreactor system에 이용하여 고과당 시럽 ( high fructose syrup )을 효과적으로 생산하기 위하여 응집방법에 의해 균체를 고정화하였다. 먼저, 응집제로 사용되는 polyethylenimine의 농도는 5%, 가교제인 glutaraldehyde의 농도는 0.2%가 적합하였다. 이 때의 응집균체량은 배양액 100㎖당 10.3g(wet weight)이었고, 응집균체의 최적 pH는 7.0, 최적 온도는 75℃ 이었다. 포도당 이성화 반응에 activitor로 작용하는 Mg^2+의 최적 농도는 0.1M이었고, 이 때의 glucose isomerase 활성은 약 40% 증가하였다. 회분식 배양에 있어서의 이성화율은 31%에 달했으며, packed-bed bioreactor의 최적 조건하에서 glucose로 부터 fructose로의 최대 이성화율은 약 40%에 달하였다.
The whole cells of Actinoplanes missouriensis KCTC 1780 which produce glucose isomerase was immobillized by flocculation method for the effective production of high fructose syrup using packed-bed bioreactor system. Amo｀ng the flocculation methods used in this study, the glucose isomerase activity of flocculated cells using 5% polyethylenimmine and 0.2% glutaraldehyde was the highest as 46.3 unit, and the flocculant was 10.3g(wet weight) per 100㎖ of broth, and the residual activity was 92.5%. In the batch operation of glucose isomerization using the flocculated cells, the optimum pH, temperature and isomerization ratio were 7.0, 75℃ and 31%, respectively. The optimum concentration of Mg^2+ which was activator on the glucose isomerization of flocculated cells was 0.1M, and glucose isomerase activity was increased by about 40% compared to none of Mg^2+. In the packed-bed bioreactor system with 1.2 hour of residence time at 70℃, the reaction stability maintained until 96 hour without loss of activity, and the equilibrium was kept up to 120 hours of the operation.
