Cordyceps militaris (C. militaris) is a unique and valuable medicinal fungus belonging to the Cordyceps species. C. militaris is the only fungus that contains cordycepin which is a biologically active compound. In previous studies, light-emitting diodes (LEDs) are known to be effective in increasing cordycepin content, but metabolic profiling of LED-stimulated C. militaris has not been confirmed. Metabolic profiling is essential to understanding the metabolic regulation of cordycepin. This study studied the physiologically active secondary metabolites of C. militaris according to the presence or absence of stimulation of LEDs through GC-MS analysis. Most of the metabolites were detected in both samples, but there was a clear difference in the detected concentration. In particular, C. militaris had a significant difference in amino acid levels when stimulated with LEDs. Our results suggested that LEDs could stimulate amino acid synthesis in C. militaris mycelium to increase the cordycepin content.

Abstract
Introduction
Materials and Methods
    1. Mycelium preparation
    2. Submerged culture
    3. Determination of cordycepin content
    4. Extraction and preparation for comprehensivemetabolite analysis
    5. GC-MS and data analysis
Results and Discussion
    1. Effect of LEDs on mycelium dry weight andcordycepin content
    2. Metabolic profiles
    3. Metabolic changes under LED stimulation
    4. Key metabolites
Acknowledgement
References

• Si Young Ha(Department of Environmental Forest Science/Institute of Agriculture and Life Science, Gyeongsang National University)
• Ji Young Jung(Department of Environmental Forest Science/Institute of Agriculture and Life Science, Gyeongsang National University)
• Jae Kyung Yang(Department of Environmental Forest Science/Institute of Agriculture and Life Science, Gyeongsang National University) Corresponding author