논문 상세보기
pp.52-58
본 연구는 고추가 함유한 효소들이 식품이 가공과 저장시에 어떠한 영향을 미칠 것인가를 추정하고자 pectinesterase(PE), polygalacturonase(PG), lipoxygenase(LOX) and peroxidase(POD)의 특성을 조사하였다. 그 결과는 다음과 같다. 1. PE는 pH 7.5에서 최대활성을 보였으며 pH 5.5이하에서는 활성이 거의 나타나지 않았다. NaCl과 CaCl_2첨가의 경우 각각 0.2M과 0.05M에서 최대활성을 보였으며, NaCl과 CaCl_2가 PE의 활성촉진제로 작용하였다. 2. PG는 pH 6.0에서 최대활성을 보였으며 알칼리성에서보다 산성에서 활성이 컸다. NaCl과 CaCl_2첨가의 경우 각각 0.3M과 0.2mM에서 최대활성을 보였으며 이후 점차 감소하여 이들 염을 첨가하지 않았을 때보다도 활성이 감소하였다. 3. LOX는 pH 7.0과 pH 8.5에서 최대활성을 보였다. 4. POD는 pH 6.0에서 최대활성을 보였고 대체로 약산성과 중성 사이에서 활성이 크게나타났다. NaCl과 CaCl_2 같은 염은 POD활성에 약간의 저해제로 사용되었다.
This study was carried out to investigate the characteristics of pectinesterase(PE), polygalacturonase(PG), lipoxygenase(LOX) and peroxidase(POD) in hot pepper to know the effect of hot pepper on food quality during food processing and storage. The results were as follows: 1. The optimum pH of PE was pH 7.5 and the activity of PE below pH 5.5 was revealed scarcely. The concentration of NaCl and CaCl_2 that showed the higest activity of PE were 0.2M and 0.05M, respectively. 2. The optimum pH of PG was pH 6.0 and the activity of PG in acidity was higher than that in alkalinity. The activity of PG was maximum at 0.3M NaCl and 0.2mM CaCl_2. Above the concentration of 0.5M NaCl and 0.5mM CaCl_2, the activity of PG was lower than that of PG not adding these salts. 3. The optimum pH of LOX was pH 7.0 and pH 8.5. 4. The optimum pH of POD was pH 6.0 and the activity of POD was higher in weak acidity and neutrality than in alkalinity. POD activity was slightly decreased by the increase of NaCl and CaCl_2 concentration.
