논문 상세보기
pp.59-68
본 연구에서는 혼합지방과 쇠기름, 옥수수기름, 들기름을 각각 15%(w/w) 수준으로 Sprague Dawley종 수컷 주에게 섭취시키면서 식이와 동시에 화학적 발암원인 DMH를 투여하여 식이지방의 조성이 대장점막 및 인지질과 phosphatidyl inositol의 지방산 조성에 미치는 영향을 살펴보았다. 대장점막과 인지질 및 PI의 지방산 조성은 식이지방의 지방산 조성에 의해 영향을 받았다. 대장점막 인지질의 C_18:2 분포는 식이중의 linoleic acid함량에 비례하는 경향을 보여 CO>BF>PO>BT군의 순서로 유의성있게 낮아졌으나 C_20:4 분포는 C_18:2 분포와는 다르게 CO군과 BT군이 같은 수준으로 높았으며 PO군이 가장 낮아 식이의 linoleic acid 함량에 꼭 비례하지는 않았다. DMH를 투여한 경우에는 PO군을 제외한 세 군에서 C_20:4 함량이 증가하였다(P<0.05). PI의 지방산 조성도 인지질과 비슷한 양상을 보여 C_18:2 분포는 CO군에서 가장 높았으며 C_20:4는 BT군이 대조군과 DMH처리군 모두에서 PO군 보다 유의성있게 높았다.
The study was designed to observe the effects of different dietary fat consumed in Korea with those of three other fats on colonic epithelial cell phospholipid and phosphatidyl inositol composition, which were known as biomarker for colon cancer. Male Sprague Dawley rats, at 7 weeks of age, were divided into control and 1, 2-dimethylhydrazine(DMH)-treated group that was again subdivided into four groups. The experimental diets contained one of four dietary fats at 15%(w/w) level, those were, blend fat(BF), beef tallow(BT), corn oil(CO) or perilla oil(PO). At the same time, each rat was injected with saline for control group or DMH twice a week for 6 weeks to give total dose of 180 mg/kg body weight. Dietary fatty acid composition influenced the fatty acid compositions of tissues. Proportions of C_18:2 colonic mucosal phospholipid well reflected dietary level of C_18:2 showing in decending CO>BF>PO>BT. The percentage of C_20:4 in phospholipid was the higher in CO and BT groups and the lowest in PO gorups. Incorporation of -linolenic acid in colonic mucosal lipid in perilla oil group was negatively correlated to the content of C_20:4. Therefore, ω3-linolenic acid rich in perilla oil could be a very important dietary source in controlling arachidonic acid level in colon epithelial cell. Therefore it could be recommend to use more perilla lil in meal preparation to reduce the risk factor against colon cancer.
