논문 상세보기
pp.172-184
The objective of this study was to determine the efficacy of a natural product of cherry tree (Prunus serrulata var. spontanea: PS) as a test substance for improving cytokine and ovalbumin-specific IgE using an ovalbumin-induced asthma disease model of 5-week-old male BALB/c mice. Lung tissue pathology was analyzed to confirm anti-inflammatory and asthmatic effects. As a result of examining the effect on changes in inflammatory cells in bronchoalveolar lavage fluid in an ovalbumin-induced asthma disease model by administering the PS sample, total cells, eosinophil, neutrophil, lymphocyte, and monocytes were significantly decreased. Concentrations of cytokine-based TNF-alpha and IL-4 and immunoglobulin E in serum were significantly increased in the asthma-inducing negative control group than in the normal group. However, high concentrations of PS decreased them. In histopathological examination of the lung tissue, it was confirmed that inflammatory cells infiltrated around the alveoli and bronchioles were increased in ovalbumin-induced asthma disease model. After administration of cherry tree extract, bronchiolar morphological changes such as mucosal thickening were slightly improved. From the above results, it was confirmed that extract of cherry tree significantly reduced inflammation expression and tissue damage in alveolar tissues. It was also confirmed that the cherry tree extract had an excellent efficacy in improving asthma inflammation.
서 론
재료 및 방법
1. 시험물질 제조
2. UPLC-Q-TOF MS를 통한 활성물질 동정
3. 실험동물 사육조건
4. 난알부민(Ovalbumin) 유도 알레르기성 기관지 천식동물모델
5. 혈액 및 시료채취
6. 기관지폐포세척액(Bronchoalveolar lavage fluid) 분리
7. 기관지폐포세척액 내의 염증세포 분석
8. 혈청 생화학 분석(Biochemistry)
9. 난알부민-특이 IgE 농도 측정
10. 사이토카인 농도 측정
11. 폐 조직의 병리학적 표본 제작, 염색 및 관찰
12. 통계 분석
결과 및 고찰
1. 추출물의 활성물질 동정
2. 일반증상 및 체중, 식이섭취량 변화
3. 기관지폐포세척액(BALF) 염증세포 측정
4. 사이토카인 농도 측정
5. 혈청 면역글로불린 E(IgE) 농도
6. 혈청 생화학 분석(Biochemistry)
7. 폐조직무게 및 조직병리검사
요약 및 결론
References
